Application of Neogambogic acid for preparing pharmaceutical for inhibiting hepatoma carcinoma cell proliferation and migration and inducing hepatoma carcinoma cell apoptosis
A technology of liver cancer cells and new gambogic acid, applied in the field of medicine, can solve the problems of poor treatment effect, short survival period, poor quality of life of liver cancer patients, etc., and achieve broad application prospects and the effect of inhibiting cell proliferation.
Inactive Publication Date: 2017-09-08
SOUTHERN MEDICAL UNIVERSITY
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Problems solved by technology
Therefore, the effect of surgery is poor, and radiotherapy and chemotherapy have obvious adverse reactions, resulting in poor quality of life and short survival period of patients with liver cancer.
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Embodiment 1
[0020] Application of neogambogic acid in preparation of drugs for inhibiting proliferation and migration of liver cancer cells and inducing apoptosis of liver cancer cells. Wherein, the above-mentioned liver cancer cells are human liver cancer SK-hep-1 cells. The effective concentration of the new xanthenic acid used in the preparation of drugs for inhibiting the proliferation of liver cancer cells is 0.75-4.5 μmol / L. The effective concentration of neoglobulinic acid used in the preparation of drugs for inhibiting the migration of liver cancer cells is 0.0625-0.5 μmol / L. The effective concentration of the new xanthenic acid used in the preparation of the drug for inducing the apoptosis of liver cancer cells is 0.03125-8 μmol / L.
[0021] In the present invention, the liver cancer cell line SK-hep-1 is conventionally cultured, and CCK-8 is used to detect the influence of different concentrations of neogambogic acid on the viability of SK-hep-1 cells; Hoechst staining is used t...
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The invention relates to an application of Neogambogic acid for preparing a pharmaceutical for inhibiting hepatoma carcinoma cell proliferation and migration and inducing hepatoma carcinoma cell apoptosis. The effective concentration of the application of the Neogambogic acid for preparing the pharmaceutical for inhibiting the hepatoma carcinoma cell proliferation is 0.75-4.5 umol / L, the effective concentration of the application of the Neogambogic acid for preparing the pharmaceutical for inhibiting the hepatoma carcinoma cell migration is 0.0625-0.5 umol / L, and the effective concentration of the application of the Neogambogic acid for preparing the pharmaceutical for inducing the hepatoma carcinoma cell apoptosis is 0.03125-8 umol / L. A hepatoma carcinoma cell system SK-hep-1 is conventionally cultivated, a CCK8 method is used, and an inverted microscope is used for detecting the effects of the Neogambogic acid with the different concentrations to the SK-hep-1 cell activity, the cell migration ability and the cell apoptosis. The experimental result adequately proves the application of the Neogambogic acid for preparing the pharmaceutical for inhibiting the hepatoma carcinoma cell proliferation and migration and inducing the hepatoma carcinoma cell apoptosis.
Description
technical field [0001] The invention relates to the technical field of medicine, in particular to the application of neogambogic acid in the preparation of drugs for inhibiting proliferation and migration of liver cancer cells and inducing apoptosis of liver cancer cells. Background technique [0002] Primary liver cancer is a common malignant tumor in my country, accounting for the second place in the death rate of various malignant tumors. Because of its insidious onset and rapid progress, it is mostly in the middle and late stage when it is discovered. Therefore, the effect of surgical treatment is poor, and radiotherapy and chemotherapy have obvious adverse reactions, resulting in poor quality of life and short survival period of patients with liver cancer. Therefore, finding low-toxic, effective and safe antineoplastic drugs is the key to the treatment of liver cancer. [0003] Comprehensive treatment based on traditional Chinese medicine is of great significance in c...
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CPCA61K31/352
Inventor 黄文华曾宝真
Owner SOUTHERN MEDICAL UNIVERSITY