Use of a hedgehog signaling pathway inhibitor in the preparation of a medicament for treating egfr overexpressed cancer
A medicament and a technology of use, applied in the field of use of Hedgehog signaling pathway inhibitors in the preparation of medicaments for the treatment of EGFR-overexpressed cancers
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Embodiment 1
[0015] Example 1: Evaluate the synergistic effect of Hedgehog inhibitor compound (I) on erlotinib on the human lung cancer NCI-H292 model with high expression of EGFR that induces resistance to erlotinib in vivo
[0016] 1 test drug
[0017] The test compound (I) was synthesized with reference to the content disclosed in CN103261198A. Preparation method: compound (I) is prepared with 0.2% Tween 80+0.5% CMC; erlotinib (erlotinib hydrochloride) is prepared with 0.2% Tween80 distilled water.
[0018] 2 experimental animals
[0019] BALB / cA-nude nude mice, 6-7 weeks old, ♀, were purchased from Shanghai Slack Experimental Animal Co., Ltd. Certificate number: SCXK (Shanghai) 2007-0005. Breeding environment: SPF grade.
[0020] 3 experimental steps
[0021] Nude mice were subcutaneously inoculated with human lung cancer NCI-H292 cells until the tumor grew to 100-250mm 3 Afterwards, the animals were randomly divided into groups (D0). See Table 1 for dosage and regimen. The tum...
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