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A kind of htnv antigen epitope linear tandem polypeptide and epitope peptide-complex tetramer and application

A technology of antigenic epitopes and epitope peptides, which is applied in the direction of fusion polypeptides, compound screening, viral antigenic components, etc., and can solve problems such as non-specific prevention and treatment methods

Active Publication Date: 2021-05-14
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

[0009] Due to the high morbidity and mortality of HFRS caused by HTNV infection, but so far there is still no specific prevention and treatment method, peptide vaccine has become a new strategy for the prevention and treatment of HTNV infection, based on the identification of HTNV-specific epitopes Based on this, the key to research is to design and synthesize HTNV epitope tandem macromolecules with strong immunogenicity

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  • A kind of htnv antigen epitope linear tandem polypeptide and epitope peptide-complex tetramer and application
  • A kind of htnv antigen epitope linear tandem polypeptide and epitope peptide-complex tetramer and application
  • A kind of htnv antigen epitope linear tandem polypeptide and epitope peptide-complex tetramer and application

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Embodiment Construction

[0042] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0043] In the present invention, the HTNV-Gn / Gc CTL 9 peptide epitope with the highest affinity of HLA-A*02 in the glycoprotein Gn / Gc sequence of the HTNV 76-118 strain and its amino acid sequence are first obtained by using a T2 cell line binding test. Further, by selecting the HTNV-Gn / Gc CTL 9 peptide epitope restricted by HLA-A*02, the B cell epitope with neutralizing activity and the general Th epitope, the HTNV antigen-based epitope was designed by using computer-aided molecular design technology. Novel tandem polypeptide antigens. By designing "B+Th+CTL" epitope tandem peptides and evaluating their immunogenicity and ability to induce specific CTL responses in transgenic mice, the effectiveness and potential application of HTNV epitope tandem peptide vaccines were evaluated value.

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Abstract

The invention provides a HTNV antigen epitope linear series polypeptide. The linear tandem polypeptide can induce specific T cell response to exert immune protection. The invention also discloses the application of the epitope linear series polypeptide in preparing polypeptide vaccine. The present invention also discloses an HLA-A*02 / epitope peptide-complex tetramer, wherein the tetramer has epitope VV9 and fluorescein PE, and can specifically bind to the corresponding TCR, thereby making the corresponding TCR can be detected by fluorescence. The invention also provides a preparation method of the tetramer and its application in the preparation of CTL detection reagents.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to an HTNV antigen epitope linear tandem polypeptide, an epitope peptide-complex tetramer and applications thereof. Background technique [0002] Hemorrhagic fever with renal syndrome (HFRS) is an acute infectious disease characterized by fever, hemorrhage, and severe renal impairment caused by bunyavirus Hantavirus (HTV) infection. Among the viral infectious hemorrhagic fever diseases, HFRS is the most widely distributed disease with the largest number of cases and great harm in the world. By the end of 2010, 1 893 555 cases had been reported in the world, and the mortality rate was as high as 15%. Among them, 1 585 942 cases were reported in my country, accounting for 83.75% of the total cases in the world. Hantaan virus (HTNV) is the prototype of the Hantaan virus genus, and it is also one of the important pathogenic microorganisms in the scope of verification of the prot...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00A61K39/12A61P31/14C12Q1/02
CPCA61K39/12C07K14/005C07K2319/00C12N2503/02C12N2760/12122C12N2760/12134G01N33/5038G01N33/505G01N2500/10
Inventor 马樱张赟袁斌唐康张宇丝张春梅方亮陈丽华杨琨金伯泉
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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