Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An impedance-type electrochemical biosensor based on electrostatic interaction, preparation method and application thereof

A biosensor and electrostatic interaction technology, applied in the field of biosensors, can solve problems such as unsustainable monitoring, achieve high sensitivity and stability, simple preparation method, and low energy consumption

Active Publication Date: 2019-10-01
ANHUI NORMAL UNIV
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it can only detect endpoints, not continuous monitoring, and even requires some complex markers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An impedance-type electrochemical biosensor based on electrostatic interaction, preparation method and application thereof
  • An impedance-type electrochemical biosensor based on electrostatic interaction, preparation method and application thereof
  • An impedance-type electrochemical biosensor based on electrostatic interaction, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Embodiment 2

Embodiment 3

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an impedance type electrochemical biosensor based on electrostatic interaction and an application thereof. The impedance type electrochemical biosensor is prepared by the steps of: modifying a gold electrode with SH-DNA; and then modifying the gold electrode with polypeptide containing lysine to prepare the electrochemical biosensor. Polypeptide containing lysine converts between electric neutrality and positive electricity by means of histone acetyltransferase and histone deacetylase, so that the polypeptide is separated from and absorbed on the surface of the electrode to induce impedance change so as to detect the histone acetyltransferase and histone deacetylase. Compared with the prior art, the preparation method of the sensor is simple and low in energy consumption. A linear relationship with the histone acetyltransferase and histone deacetylase is constructed by means of electrochemical impedance change of the electrode, so that two enzymes are detected. Therefore, the sensor has relatively high sensitivity and stability.

Description

technical field The invention belongs to the technical field of biosensors, and in particular relates to an electrostatic-based impedance electrochemical biosensor, a preparation method and an application thereof, which can be used for detection of acetylase and sirtuin. Background technique Protein acetylation, that is, the covalent modification of protein acetyl group, is an important post-translational modification mechanism in the regulation of eukaryotic cells, which can regulate the interaction between proteins in cells, protein stability and signal transduction , in order to regulate physiological responses and disease progression. In the acetylation of different kinds of proteins, lysine acetylation exists as the main post-translational modification in eukaryotes and prokaryotes. Lysine acetylation is a typical "histone code", which is closely related to transcription activity, histone deposition, DNA replication and DNA repair. Histone acetylases transfer the ace...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/48G01N27/02G01N27/327
Inventor 王广凤杨新胜
Owner ANHUI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com