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Application of rheum emodin in preparation of drugs for killing in-vitro parasitic ciliates of fish

A technology of emodin and ciliates, which is applied in the field of killing parasites and fishery medicines, can solve the problems of environmental pollution, high residue, carcinogenicity, etc., and achieve the effect of rich sources, simple extraction and preparation process, and broad application prospects

Active Publication Date: 2017-09-15
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because these chemicals have high toxicity, high residue, carcinogenic, teratogenic, mutagenic and environmental pollution hazards, they have been banned in the production of edible fish

Method used

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  • Application of rheum emodin in preparation of drugs for killing in-vitro parasitic ciliates of fish
  • Application of rheum emodin in preparation of drugs for killing in-vitro parasitic ciliates of fish
  • Application of rheum emodin in preparation of drugs for killing in-vitro parasitic ciliates of fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: The effect of emodin on the infective larvae of the multi-seed melon worm

[0021] (1) Drug preparation: Weigh 1.5 mg of emodin powder, dissolve it with 20 μL of absolute ethanol, add distilled water to prepare a stock solution with a concentration of 1280 mg / L, and dilute to 64, 32, 16 , 8, 4, 2, 1, 0.5 and 0 mg / L (control). Taking the content of absolute ethanol (0.08%) in the highest concentration medicinal liquid used in the experiment as a standard, a control solution containing 0.08% absolute ethanol was prepared.

[0022] (2) Take a 96-well plate, add 100 μL of worm solution containing about 200 larvae to each well, and then add 100 μL of different concentrations of drug solution to each well, so that the final drug concentration is 32, 16, 8, 4, 2 , 1, 0.5, 0.25 and 0 mg / L (control), each drug concentration was repeated 5 times. The experimental temperature was maintained at 23±0.5°C, and the lethal time of larvae in different drug concentrations...

Embodiment 2

[0025] Embodiment 2: The killing effect of emodin to the adults of the multi-seed melon worm

[0026] (1) The preparation of emodin medicine: with embodiment 1.

[0027] (2) Take a 24-well plate, add 200 μL of aqueous solution containing about 60 adults to each well, and accurately count the number of adults in each well under a 4-fold microscope, then add 200 μL of different concentrations of liquid medicine to each well, so that The final concentrations of the drug solution were 32, 16, 8, 4, 2, 1, 0.5, 0.25 and 0 mg / L in sequence, and three parallels were set for each concentration. The experimental temperature was 23±0.5°C. Observe and record the death time of adults in 5h and the mortality rate of the 5th hour under a 4-fold microscope, calculate the 5h lethality and the 5h half effective concentration (EC 50 ) (see Table 3). Surviving adults will form cysts and continue to hatch. On the 22nd hour of the experiment, after the cysts are completely hatched, count the num...

Embodiment 3

[0031] Embodiment 3: The killing effect of emodin medicine on the cyst of the multi-seed melon worm

[0032] (1) The preparation of emodin medicine: with embodiment 1.

[0033] (2) Add 200 μL of liquid containing about 40 adults into each well of a 24-well plate, and then place the 24-well plate at a temperature of 23±0.5°C for 6 hours. After cysts are formed, accurately count the number of cysts in each well , and then add 200 μL of different concentrations of the drug solution to each well, so that the final concentration of the drug solution is 32, 16, 8, 4, 2, 1, 0.5, 0.25 and 0 mg / L, and each concentration is set in 3 parallels. Then continue to stand at 23±0.5°C for 16 hours. After the cysts are completely hatched, count the average number of hatched larvae per cyst. The calculation formula is as follows:

[0034] The average number of hatched larvae per cyst = the number of larvae in each well / the number of cysts in each well

[0035] The results showed that the cysts...

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Abstract

The invention discloses an application of rheum emodin in preparation of drugs for killing in-vitro parasitic ciliates of fish, relates to the technical field of fishery medicines, particularly relates to an application in preparation of drugs for killing ichthyophthirius multifilis, and belongs to the technical field of drugs for killing parasites. The rheum emodin is applied to preparation of drugs for killing in-vitro parasitic ciliates of fish for the first time, and is especially applied to killing ichthyophthirius multifilis. The rheum emodin is obtained from a natural plant, and is free of residue, free of pollution, free of public hazard and nontoxic to a human body, so that the rheum emodin is easily degraded in the environment, one-time killing of the parasites can be achieved within an effective dose range, and the effect of controlling ichthyophthiriasis is achieved. The medial lethal concentration of the rheum emodin on the fish is 17.8 times and 7.0 times of the effective concentration of killing larvae and adults of the ichthyophthirius multifilis separately; and the rheum emodin is safe to the fish within an effective dose range of an insecticide. The rheum emodin is abundant in source, the extraction preparation process is simple, and the rheum emodin has a wide application prospect in development of a novel environment-friendly fishery medicine for controlling the in-vitro parasitic ciliates of the fish.

Description

technical field [0001] The invention relates to the technical field of fishery medicines, belongs to the technical field of parasite-killing medicines, and relates to a new application of emodin, in particular to an application of emodin in the preparation of medicines for killing fish ectoparasitic ciliates. Background technique [0002] Parasitic ciliates are one of the most serious diseases that harm aquaculture, especially the multi-seeded cilia. The disease has a wide range of distribution, no host specificity, rapid onset and high mortality, causing huge economic losses to aquaculture. For a long time, malachite green and mercury preparations and other chemical drugs have been used for the prevention and treatment of multi-seeded melon worm disease. Because these chemicals have high toxicity, high residue, carcinogenic, teratogenic, mutagenic and environmental pollution hazards, they have been banned in the production of edible fish. Therefore, there is an urgent nee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P33/14
CPCA61K31/122
Inventor 张其中周生玉
Owner JINAN UNIVERSITY
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