Deodorizing microbial agent and preparation method thereof

Abstract

The invention belongs to the field of environmental protection and particularly discloses aa microbial deodorant for deodorization. The microbial deodorant comprises the active components: a microbial agent, molasses, amino acids and vitamins; the microbial agent is a composite bacterial agent of Kluyveromyces marxianus and Bacillus subtilis; the microbial deodorant is capable of inhibiting the growth of putrefying bacteria, improving the degrading means of organic matters and decreasing the release of ammonia and hydrogen sulfide and occurrence of ammonia matters, and is capable of deceasing methane from bacterial decomposition, thereby arriving at deodorization; the materials of the microbial deodorant are easy to obtain, the preparation method is simple, and the microbial deodorant is worthy of popularization and application.

Description

technical field

[0001] The invention belongs to the field of environmental protection, and specifically relates to a microbial bacterial agent for deodorizing biological sewage and an application thereof, especially a microbial bacterial agent suitable for treating stinky domestic sewage. Background technique

[0002] With the rapid development of economy and society and the continuous acceleration of urbanization, indoor and outdoor environmental pollution is always becoming more and more prominent. People's attention to environmental pollution has shifted from outdoor to indoor. An important cause of pollution is the massive discharge of urban wastewater. According to According to statistics, the total discharge of urban wastewater in my country is about 34.5 billion tons per year, of which domestic sewage accounts for more than 30%. COD and BOD in domestic sewage 5 Seriously exceeded. A kind of bad smell is emitted in the family bathroom and the kitchen sewer due to vario...

Examples

Embodiment 2

[0037] A microbial deodorant prepared by the following preparation method:

[0038] (1) Preparation of Kluyveromyces marx fermentation broth: activate Kluyveromyces marx and inoculate it in the liquid medium for yeast culture, cultivate it in a shaker at 200 r / min for 48 h at 28 °C, and count Then adjust the concentration of Kluyveromyces marx to 1 × 10 with sterile water 7 CFU / mL;

[0039] (2) Preparation of Bacillus subtilis fermentation broth: Inoculate Bacillus subtilis into the bacterial culture medium, culture at 35 °C in a shaker at 200 r / min for 36 h, after counting, adjust the concentration of Bacillus subtilis with sterile water Concentration to 1×10 7 CFU / mL;

[0040] (3) Mix the fermentation liquid of step (1) and step (2) according to the volume ratio of 0.4:1;

[0041] (4) Add molasses, glycine, aspartic acid, and vitamin B12 to the mixed species of fermentation broth, and adjust their concentrations to 0.3 mg / mL, 0.3 mg / mL, 0.3 mg / mL, and 0.3 mg / mL to obtain...

Embodiment 3

[0043] A microbial deodorant prepared by the following preparation method:

[0044] (1) Preparation of Kluyveromyces maximus fermentation broth: Activate Kluyveromyces maxima and inoculate it in the liquid medium for cultivating yeasts, culture it in a shaker at 160 r / min at 28 °C for 72 hours, and after counting Adjust the concentration of K. marx to 1 x 10 with sterile water. 9 CFU / mL;

[0045] (2) Preparation of Bacillus subtilis fermentation broth: Inoculate Bacillus subtilis into the bacterial culture medium, culture at 30 °C in a shaker at 160 r / min for 42 h, after counting, adjust the concentration of Bacillus subtilis with sterile water Concentration to 1×10 7 CFU / mL;

[0046] (3) Mix the fermentation liquid of step (1) and step (2) according to the volume ratio of 1.1:1;

[0047](4) Add molasses, glycine, aspartic acid, serine, and vitamin B12 to the mixed species of fermentation broth, and adjust their concentrations to 0.3 mg / mL, 0.2 mg / mL, 0.2 mg / mL, 0.2 mg / mL,...

technical field

[0001] The invention belongs to the field of environmental protection, and specifically relates to a microbial bacterial agent for deodorizing biological sewage and an application thereof, especially a microbial bacterial agent suitable for treating stinky domestic sewage. Background technique

[0002] With the rapid development of economy and society and the continuous acceleration of urbanization, indoor and outdoor environmental pollution is always becoming more and more prominent. People's attention to environmental pollution has shifted from outdoor to indoor. An important cause of pollution is the massive discharge of urban wastewater. According to According to statistics, the total discharge of urban wastewater in my country is about 34.5 billion tons per year, of which domestic sewage accounts for more than 30%. COD and BOD in domestic sewage 5 Seriously exceeded. A kind of bad smell is emitted in the family bathroom and the kitchen sewer due to vario...

Examples

Embodiment 2

[0037] A microbial deodorant prepared by the following preparation method:

[0038] (1) Preparation of Kluyveromyces marx fermentation broth: activate Kluyveromyces marx and inoculate it in the liquid medium for yeast culture, cultivate it in a shaker at 200 r / min for 48 h at 28 °C, and count Then adjust the concentration of Kluyveromyces marx to 1 × 10 with sterile water 7 CFU / mL;

[0039] (2) Preparation of Bacillus subtilis fermentation broth: Inoculate Bacillus subtilis into the bacterial culture medium, culture at 35 °C in a shaker at 200 r / min for 36 h, after counting, adjust the concentration of Bacillus subtilis with sterile water Concentration to 1×10 7 CFU / mL;

[0040] (3) Mix the fermentation liquid of step (1) and step (2) according to the volume ratio of 0.4:1;

[0041] (4) Add molasses, glycine, aspartic acid, and vitamin B12 to the mixed species of fermentation broth, and adjust their concentrations to 0.3 mg / mL, 0.3 mg / mL, 0.3 mg / mL, and 0.3 mg / mL to obtain...

Embodiment 3

[0043] A microbial deodorant prepared by the following preparation method:

[0044] (1) Preparation of Kluyveromyces maximus fermentation broth: Activate Kluyveromyces maxima and inoculate it in the liquid medium for cultivating yeasts, culture it in a shaker at 160 r / min at 28 °C for 72 hours, and after counting Adjust the concentration of K. marx to 1 x 10 with sterile water. 9 CFU / mL;

[0045] (2) Preparation of Bacillus subtilis fermentation broth: Inoculate Bacillus subtilis into the bacterial culture medium, culture at 30 °C in a shaker at 160 r / min for 42 h, after counting, adjust the concentration of Bacillus subtilis with sterile water Concentration to 1×10 7 CFU / mL;

[0046] (3) Mix the fermentation liquid of step (1) and step (2) according to the volume ratio of 1.1:1;

[0047](4) Add molasses, glycine, aspartic acid, serine, and vitamin B12 to the mixed species of fermentation broth, and adjust their concentrations to 0.3 mg / mL, 0.2 mg / mL, 0.2 mg / mL, 0.2 mg / mL,...

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