Method for rapidly establishing gestational-diabetes-mellitus mouse animal model

Abstract

The invention discloses a method for rapidly establishing a gestational-diabetes-mellitus mouse animal model. The method includes the following steps that (1) 7-week female mice are fed with a high-fat diet, the female mice were mated with male mice after one week, vaginal plugs are viewed, the time of pregnance is registered as 0.5 day on the day when the vaginal plugs appear, an oral glucose tolerance test (OGTT) is carried out for excluding pregestational diabetes mellitus, and the weight is recorded; (2) the mice continue to be fed with the high-fat diet, the OGTT is carried out again on the day 16.5 of pregnance, and compared with mice fed with standard feed, the glucose tolerance of a model group is reduced, and the model is successful. The mouse model which is most similar to human GDM, stable, reliable, short in molding time, low in economic cost, high in practicability, capable of copying, remarkable in phenotype and capable of conforming to clinical characteristics of the human GDM.

Description

Technical field [0001] The invention belongs to the field of pharmacology, and specifically is a method for quickly constructing a mouse animal model of gestational diabetes. Background technique [0002] Diabetes that has normal glucose metabolism or potential impaired glucose tolerance before pregnancy, appears or is diagnosed during pregnancy, is also called "gestational diabetes (GDM)". In the middle and late stages of pregnancy, the insulin resistance of pregnant women increases, such as placental lactogen, estrogen, progesterone, cortisol, and placental insulinase, which decrease the sensitivity of pregnant women to insulin as the gestational age increases. In order to maintain normal glucose metabolism levels, insulin requirements must be increased accordingly. For pregnant women with restricted insulin secretion, this physiological change cannot be compensated during pregnancy and the blood sugar will rise, which will aggravate the original diabetes or develop GDM. Most...

AI Technical Summary

Problems solved by technology

The most widely used type is the induced type, and the most popular ones are the streptozotocin (STZ)-induced diabetes model and the high-fat-induced obesity model. The principle of the former is that the islet β-cell toxicity of STZ drugs on experimental animals damages the islet β-cell , causing insufficient insulin secretion, causing a phenotype similar to type 1 diabetes (insulin secretion deficiency), and the weight of fetal mice decreased, which did not meet the characteristics of GDM; Pre-obese or type 2 diabetes, after mating, create a mouse model of obesity or type 2 diabetes combined with pregnancy, not the first diabetes after pregnancy, which does not meet the characteristics of GDM

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