Preparation method and use of eurycoma longifolia total-alkaloid extract

A technology of total alkaloids and extracts, which is applied in the field of new pharmaceutical applications of Dongge Ali total alkaloids extracts, can solve problems such as unclear active ingredients and mechanisms, cardiovascular adverse reactions, and unacceptable patients, and achieve good industry The effect of improving the prospects of globalization, improving the compliance of medication, and reducing the dosage

Inactive Publication Date: 2017-09-22
JIANMIN PHARMA GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the active ingredients and mechanism of Tongkat Ali’s anti-uric acid effect are still unclear, and the treatment is still based on very traditional methods of taking medicine such as decoction in water. Due to the extremely bitter taste of Tongkat Ali, it is difficult for patients to accept large doses of medicine. At the same time, This way of taking the drug also has relatively large side effects, often leading to high libido and other cardiovascular adverse reactions in patients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Extraction of total alkaloids: cut 5Kg of Dongge Ali into thin slices, add 8 times the weight of 70% (weight concentration) ethanol to reflux and extract 3 times, each time for 1 hour (soak for 50 minutes before the first extraction), filter, and combine Three times filtrate, decompression reclaims ethanol to no alcohol smell, after cooling, adjust the pH of the extract with 2% (weight concentration) sulfuric acid to be 2, let stand, centrifuge, get the supernatant and adjust the pH to be 10 with ammonia water, then add 2 Double the volume of chloroform and extract three times, combine the chloroform layers, recover the chloroform and evaporate to dryness to obtain 0.36Kg of the crude extract of total alkaloids;

[0022] 2) Purification of total alkaloids: dissolve the crude extracts of total alkaloids with a small amount of ethanol, and put them on a CG161 type reversed-phase polymer chromatography packed column (polystyrene resin, specific surface area 900m 2 / g, a...

Embodiment 2

[0024] 1) Extraction of total alkaloids: Cut 5Kg of Dongge Ali into thin slices, add 10 times the weight of 50% ethanol to reflux and extract twice, each time for 2 hours, extract the ethanol under reduced pressure and recover the ethanol until it has no alcohol smell, let it cool and use dilute Adjust the pH to 4 with sulfuric acid, let stand, centrifuge, adjust the pH to 12 with ammonia water in the supernatant, then add 4 times the volume of chloroform to extract twice, recover the solvent and evaporate to dryness to obtain 0.45 g of crude extract of total alkaloids;

[0025] 2) Purification of total alkaloids: dissolve the crude extracts of total alkaloids in ethanol, put on CG300 type reversed-phase polymer chromatography packed column (polystyrene resin, specific surface area 700m2 / g, average pore size 300A, Mitsubishi Chemical), and then Elute with water, 20% ethanol, and 70% ethanol, collect the 70% ethanol eluate, recover the ethanol and concentrate to a thick paste, v...

Embodiment 3

[0027] 1) Extraction of total alkaloids: Cut 5Kg of Dongge Ali into thin slices, add 5 times the weight of 90% ethanol to reflux and extract twice, each time for 1 hour, extract the ethanol under reduced pressure and recover the ethanol until it has no alcohol smell, and use it after cooling Adjust the pH to 1 with dilute sulfuric acid, let it stand, and centrifuge, adjust the pH of the supernatant to 8 with ammonia water, then add 1 times the volume of chloroform to extract 5 times, recover the solvent and evaporate to dryness to obtain 0.31Kg of the crude extract of total alkaloids;

[0028] 2) Purification of total alkaloids: dissolve the crude extracts of total alkaloids in ethanol, put them on MCI CHP20P type reversed-phase polymer chromatography packed column (polystyrene resin, average particle size 120 μm, pore size 45 nm, Mitsubishi Chemical), and water , 50% ethanol, 90% ethanol elution, collect 90% ethanol eluate, reclaim ethanol and concentrate to thick paste, vacuu...

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PUM

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Abstract

The invention discloses a preparation method of eurycoma longifolia total-alkaloid extract. The method includes the two steps of total alkaloid extraction and upper reversed-phase polymer chromatography packed column purification, and belongs to the field of pharmacy. The prepared eurycoma longifolia total-alkaloid extract can promote excretion of creatinine in urine of rats, reduce the activity of xanthine oxidase in the liver, meanwhile reduce the content of uric acid, urea nitrogen and creatinine in serum of the rats, and improve the content of uric acid in the urine of the rats. It is shown that the eurycoma longifolia total-alkaloid extract has significant effects of reducing uric acid and promoting the excretion of creatinine and can be used for preparing an anti-uric-acid drug.

Description

technical field [0001] The invention relates to a preparation method of the total alkaloid extract of Dongge Ali and a new pharmaceutical application of the total alkaloid extract of Dongge Ali, belonging to the field of pharmacy. Background technique [0002] Hyperuricemia refers to the increase of serum uric acid level or the deposition of urate in the kidney caused by uric acid metabolism disorder, and the clinical manifestation is mainly gout syndrome. In recent years, the incidence of hyperuricemia has been increasing worldwide. Currently, the global prevalence of hyperuricemia is as high as 10% to 25%. Disorders of purine metabolism and decreased urate excretion are the direct causes of hyperuricemia. Hyperuricemia can directly lead to the onset of gout, and also promote the onset of diseases such as hyperlipidemia, hypertension, obesity, and insulin resistance. Therefore, hyperuricemia has posed a serious threat to human health. Studies have shown that Tongkat Ali ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P19/06
Inventor 黄志军朱立彬陈骞曾庆恢李霞翟莉向阳陈鹏
Owner JIANMIN PHARMA GRP CO LTD
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