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Simple method for screening or identifying imazapic-resistant rice

A technology for resisting methazepam and methazepam, applied in botany equipment and methods, applications, plant genetic improvement, etc., can solve problems such as many technical links, time-consuming and laborious, and affect the accuracy of results, and achieve good technology effect of means

Inactive Publication Date: 2017-09-29
JIANGSU XUHUAI DISTRICT HUAIYIN AGRI SCI RES INST
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is time-consuming and labor-intensive, and the concentration and uniformity of spraying are not easy to control, which affects the accuracy of the results.
However, the method of using molecular markers is relatively expensive and has many technical links.

Method used

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  • Simple method for screening or identifying imazapic-resistant rice

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Experimental program
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Effect test

Embodiment

[0015] The tested varieties were 2 non-herbicide-resistant japonica rice varieties (Huaidao 5 and Huaidao 119) and three new methazepam-resistant germplasms (T1, T2, T3). Choose 2 portions of plump rice seeds for each variety, 100 grains per portion, put them into white sulfuric acid paper bags, and seal them with a stapler or paper clip. One was immersed in the dilute solution of the herbicide methazapyr, and the other was soaked in water as a control. Germination rate was counted after sowing and germination (Table 1), and the results showed that after soaking the seeds with the diluted solution of memazazole, the germplasm germplasm rates of T1, T2, and T3 were above 85%, which was the same as that after seed soaking in clear water (CK). The germination rates of Huaidao 5 and Huaidao 119 varieties were 2% and 0% after soaking with imazethapyr, respectively, and the germination rates of the control reached 96% and 95%, respectively. It showed that the herbicide-resistant va...

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Abstract

The invention discloses a method for conveniently screening or identifying methazoprase-resistant rice, which comprises the following steps: purchasing the herbicide Bailongtong, diluting it with water until the concentration of methazepam is 0.1ml / L, drying the tested variety Put the grains into a water-absorbing sulfuric acid paper bag or a gauze bag, immerse in the diluent, and take it out after 48 hours; soak the seeds in clean water after rinsing, take out the water after 24 hours, and sow them evenly in a plastic plug with a 1cm rice seedling substrate, covering After 1cm of the same substrate, pour water to keep it moist, keep the room temperature at 22-30°C, and observe and count after 5-7 days. The invention can screen or identify the rice resistant to the herbicide methazepam simply, quickly, efficiently and accurately, and provides a good technical means for the breeding of the rice resistant to the herbicide methazepam.

Description

technical field [0001] The invention relates to the field of rice breeding, in particular to a method for simply screening or identifying methazepam-resistant rice. Background technique [0002] Rice is the main food crop that more than half of the world's population depends on, and it is of great significance to human life and food security. my country has a long history of planting rice and has a vast territory. It is one of the main rice producing countries in the world. The annual planting area is about 35 million hm 2 , accounting for about 29% of the sown area of ​​food crops. A large number of weeds have always occurred in rice fields in all rice-growing areas of our country. According to statistics, there are more than 200 species of weeds in paddy fields across the country, among which there are about 40 species of weeds that occur commonly, cause serious damage, and are the most common (10 to 20 species are distributed in various rice areas). According to the s...

Claims

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Application Information

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IPC IPC(8): A01H1/04
CPCA01H1/04
Inventor 李刚
Owner JIANGSU XUHUAI DISTRICT HUAIYIN AGRI SCI RES INST