Method for positioning target gene of hyriopsis cumingii mantle
A technology of triangular sailfish and positioning method, which is applied in the biological field, can solve problems such as genetic difficulties, and achieve the effects of good technical adaptability, improvement and improvement of pearl quality, and easy operation
Active Publication Date: 2014-12-03
SHANGHAI OCEAN UNIV
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Problems solved by technology
[0003] At present, the research on the formation mechanism of shells and pearls is mainly focused on the isolation of proteins and polypeptides involved in mineralization and the cloning of their genes, but there are still many difficulties in the study of the functions of the genes
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Embodiment 1
[0043] The solute of the 4% paraformaldehyde solution is paraformaldehyde, the solvent is PBS, and the final concentration of paraformaldehyde is 4% (mass percentage). The preparation method of described PBS is: NaCl 8 g, KCl 0.2 g, NaCl 2 HPO 4 1.44 g, KH 2 PO 4 0.24g, with DEPC-H 2 O with 1 L, adjust the pH value to 7.4. The DEPC-H 2 The preparation method of O was as follows: 1 mL DEPC was added to 1000 mL double-distilled water, stirred overnight with a magnetic stirrer, autoclaved, and sealed for later use.
[0044] The working concentration of Triton X-100 is 0.3%, and the solvent is PBS. 0.2% glycine solution, the solute is glycine, the solvent is PBS, and the final concentration of glycine is 0.2% (mass percentage). The specific components of NTE Buffer are: 500 mmol / L NaCl, 10 mmol / L Tris, 1 mmol / L EDTA, pH 8.0. The preparation method of 1% BSA solution is as follows: 0.5 g BSA is dissolved in 50 mL Blocking solution.
[0045] The triangular sail mussel is a...
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Abstract
The invention discloses a method for positioning a target gene of a hyriopsis cumingii mantle. The method comprises the steps as follows: carrying out in-situ hybridization on hyriopsis cumingii mantle tissues, and positioning the target gene according to an in-situ hybridization result of the hyriopsis cumingii mantle tissues, wherein a probe used for in-situ hybridization of the hyriopsis cumingii mantle tissues is a probe aiming at the target gene, the sequence of the target gene is as shown in SEQ ID No.1, and the sequence of the probe is as shown in SEQ ID No.2. The gene positioning method is provided for shell formation and pearl cultivation study in which the hyriopsis cumingii mantle involves, and a technical means is also provided for further study on various molecular mechanisms in the growth and pearl cultivation processes of hyriopsis cumingii. The method is easy to operate, time-saving, free from special equipment and low in cost, and has an important meaning on study on improvement of fresh water pearl quality in cultivation.
Description
technical field [0001] The invention relates to the field of biological technology, in particular to a positioning method of a target gene in the mantle of the triangular sail mussel. Background technique [0002] Triangle sail mussel is the main high-quality freshwater pearl mussel in my country, which has important economic value. The mantle of the pearl-growing clam can secrete substances to form shells, and it can also secrete nacre to form pearls. The shell of freshwater mussels can usually be divided into three layers: cuticle, prismatic layer and nacre, and the tissue cells in different parts of the mantle have the function of forming different shell structures. It is generally believed that the stratum corneum is secreted by the shell protrusions at the edge of the mantle, the prismatic layer is secreted by the tall columnar cells of the mantle marginal membrane, and the nacre is secreted by the columnar cells of the central mantle membrane. High-quality freshwater...
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IPC IPC(8): C12Q1/68
Inventor 李家乐李西雷白志毅汪桂玲罗红瑞
Owner SHANGHAI OCEAN UNIV