Method for multi-parameter detection of ocular irritation by using in-vitro animal cornea model

An irritating, multi-parameter technology that is used in biochemical equipment and methods, microbial determination/inspection, measurement devices, etc., and can address differences in responses between animals and humans, multiple eye irritation in vitro methods that are not approved, and low throughput And other issues

Inactive Publication Date: 2017-10-13
程树军 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of traditional animal experiments include: the subjectivity of the method, the difference in the response of animals and humans, the long cycle, low throughput, and the use of live animals to cause painful death of animals, etc.
However, in practice, you may be exposed to a variety of exogenous chemicals, and re

Method used

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  • Method for multi-parameter detection of ocular irritation by using in-vitro animal cornea model
  • Method for multi-parameter detection of ocular irritation by using in-vitro animal cornea model
  • Method for multi-parameter detection of ocular irritation by using in-vitro animal cornea model

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0125] Specific Example 1 Prediction of eye irritation under multiple exposures of a cosmetic set based on cow cornea detection

[0126] 1. Preparation of experimental materials

[0127] 1.1 Preparation of solution

[0128] 1.1.1 HBSS solution: 1 package of HBSS powder (purchased from Sigma), add 0.35g sodium bicarbonate (domestic analytical grade), add 1L ultrapure water, mix well, adjust the pH to 7.2-7.3 with hydrochloric acid, filter 0.22μm Membrane filtration sterilization, adding penicillin (100IU / mL) and streptomycin (100IU / mL), 4 ℃ refrigerator can be stored for 4 weeks.

[0129] 1.1.2 MEM medium: 1 package of MEM medium powder (purchased from Sigma), add 2.2g sodium bicarbonate (domestic analytical grade) and 0.292g L-glutamine (purchased from Sigma), add 1L ultrapure water, fully Mix well, adjust the pH to 7.2-7.3 with hydrochloric acid, filter and sterilize with a 0.22μm filter membrane, and store in a refrigerator at 4°C for 4 weeks. The preparation method for phenol red...

Embodiment 2

[0190] Example 2 Prediction of eye irritation under multiple exposures of a certain brand of eye drops based on cow cornea detection

[0191] 1. Preparation of experimental materials

[0192] 1.1 Preparation of solution

[0193] 1.1.1 HBSS solution: 1 package of HBSS powder (purchased from Sigma), add 0.35g sodium bicarbonate (domestic analytical grade), add 1L ultrapure water, mix well, adjust the pH value to 7.2-7.3, 0.22μm with hydrochloric acid Sterilize by filtration, add penicillin (100IU / mL) and streptomycin (100IU / mL), and store in a refrigerator at 4°C for 4 weeks.

[0194] 1.1.2 MEM medium: 1 package of MEM medium powder (purchased from Sigma), add 2.2g sodium bicarbonate (domestic analytical pure) and 0.292g L-glutamine (purchased from Sigma), add 1L ultrapure water, Mix well, adjust the pH to 7.2-7.3 with hydrochloric acid, filter and sterilize with 0.22μm filter membrane, and store in a refrigerator at 4°C for 4 weeks. The preparation method for phenol red and without p...

Embodiment 3

[0261] Example 3 Prediction of eye irritation under multiple exposures of a certain brand of invisible eye care solution based on cow cornea

[0262] 1. Preparation of experimental materials

[0263] 1.1 Preparation of solution

[0264] 1.1.1 HBSS solution: 1 package of HBSS powder (purchased from Sigma), add 0.35g sodium bicarbonate (domestic analytical grade), add 1L ultrapure water, mix well, adjust the pH value to 7.2-7.3, 0.22μm with hydrochloric acid Sterilize by filtration, add penicillin (100IU / mL) and streptomycin (100IU / mL), and store in a refrigerator at 4°C for 4 weeks.

[0265] 1.1.2 MEM medium: 1 package of MEM medium powder (purchased from Sigma), add 2.2g sodium bicarbonate (domestic analytical pure) and 0.292g L-glutamine (purchased from Sigma), add 1L ultrapure water, Mix well, adjust the pH to 7.2-7.3 with hydrochloric acid, filter and sterilize with 0.22μm filter membrane, and store in a refrigerator at 4°C for 4 weeks. The preparation method for phenol red and w...

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PUM

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Abstract

The invention discloses a method for the multi-parameter detection of ocular irritation by using an in-vitro animal cornea model. The method comprises the following steps: 1) preparing and keeping an in-vitro animal cornea; 2) exposing a test substance; concentration of a test substance, and determining the concentration of the test substance, the exposure time, and the post-incubation time; 3) selecting the evaluation parameters of the test substance; 4) measuring the evaluation parameters; 5) evaluating and predicting the ocular irritation of the test substance. The in-vitro animal cornea is similar to a human cornea and has normal physiological activity after undergoing short-time in vitro keeping maintenance, and the multiple evaluation parameters are measured and analyzed after the test substance is in contact with the cornea in order to evaluate the influences of the test substance on the cornea after repeated/multiple exposure, and the potential ocular irritation is predicted. The method disclosed in the invention can replace traditional Draize rabbit eye test, can be directly used to compare, evaluate and predict the potential ocular irritation of daily chemical products, chemicals, medicines and other products/raw materials/formulas, and has the advantages of high flux, low cost and short time.

Description

Technical field [0001] The invention relates to a method for detecting eye irritation using multiple parameters of an isolated animal cornea model. Background technique [0002] Human eyes may be exposed to one or more substances such as cosmetics, drugs, pesticides, and environmental pollutants. Subjective or accidental exposure to these foreign substances may cause health risks to the population. Therefore, assessing the potential eye irritation of the substances that may be exposed to is of great significance for protecting the health of the population. In the 1830s, untested eyelash products containing p-phenylenediamine were allowed to be sold in the US market, leading to adverse reactions such as eye allergies, corneal ulcers and blindness caused by consumers using such products. This public hazard incident prompted the US Food, Drug and Cosmetics Administration (FDA) to take action in 1938, mandating the safety of eye contact products. [0003] For the toxicological evalua...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5008G01N33/5044
Inventor 程树军秦瑶黄健聪
Owner 程树军
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