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Active polysaccharide protein of Morchella, Polygonatum and Snowdrop, and preparation method and application thereof

A technology of active polysaccharide and Morchella, which is applied in the preparation methods of peptides, chemical instruments and methods, peptide/protein components, etc., and can solve problems such as the lack of unified understanding.

Active Publication Date: 2021-04-20
秦小波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, how to obtain polysaccharide proteins with important medicinal activities from some traditional Chinese medicines has not yet formed a unified understanding

Method used

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  • Active polysaccharide protein of Morchella, Polygonatum and Snowdrop, and preparation method and application thereof
  • Active polysaccharide protein of Morchella, Polygonatum and Snowdrop, and preparation method and application thereof
  • Active polysaccharide protein of Morchella, Polygonatum and Snowdrop, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 10 g of Morchella, Rhizoma Rhizoma Polygonatum, and snow lotus dry materials and mix a total of 30 g, pulverize to 300 mesh with a superfine pulverizer, then add 0.1M sodium chloride solution with a mass-volume ratio of 15 times the volume, stir evenly, and extract 6 hours, filtered through two layers of gauze, and centrifuged (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0036] Take the above precipitate, add 0.5M sodium chloride solution with a mass volume ratio of 5 times the volume, stir evenly, extract for 2 hours, filter through two layers of gauze, and centrifuge (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0037] All the obtained supernatants were combined to obtain an extract.

[0038] Add sulfuric acid to the extract to reach 40% saturation, let it stand for 5 hours, and centrifuge (4°C, 10,000g, 20min) to remove the precipitate;

[0039] Take the supernatant, use ultrafiltration to concentrate and desalt, the mol...

Embodiment 2

[0042] Get 20g of hickory chick and sealwort dried material respectively and mix 40g altogether, pulverize to 300 mesh with superfine pulverizer, then add the 0.3M sodium chloride solution of mass volume ratio 10 times of volume, stir well, leaching 6 hours, 2 Filter through a layer of gauze and centrifuge (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0043] Take the above precipitate, add 0.3M sodium chloride solution with a mass volume ratio of 8 times the volume, stir evenly, extract for 4 hours, filter through two layers of gauze, and centrifuge (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0044] All the obtained supernatants were combined to obtain an extract.

[0045] Add sulfuric acid to the extract to reach 40% saturation, leave it for 6 hours, and centrifuge (4°C, 10,000g, 20min) to remove the precipitate;

[0046] Take the supernatant, use ultrafiltration to concentrate and desalt, the molecular weight cut-off of the ultrafil...

Embodiment 3

[0049]Get 50g of hickory chick dry material, pulverize to 300 order with superfine pulverizer, then add the 0.2M sodium chloride solution of mass volume ratio 20 times of volume, stir, leaching 4 hours, 3 layers of gauze filter, centrifugal ( 4°C, 5,000g, 30min), to obtain supernatant and precipitate.

[0050] Take the above precipitate, add 0.2M sodium chloride solution with a mass volume ratio of 10 times the volume, stir evenly, extract for 3 hours, filter with 3 layers of gauze, and centrifuge (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0051] All the obtained supernatants were combined to obtain an extract.

[0052] Add sulfuric acid to the extract to reach 40% saturation, let it stand for 4 hours, and centrifuge (4°C, 10,000g, 20min) to remove the precipitate;

[0053] Take the supernatant, use ultrafiltration to concentrate and desalt, the molecular weight cut-off of the ultrafiltration membrane is 5000 Dalton; the volume is concentrated to 30% of...

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Abstract

The invention provides a preparation method of active polysaccharide protein, which comprises the following steps: (1) ultrafinely pulverize the raw material to 300 mesh, then add sodium chloride solution for leaching, filter and centrifuge, and obtain the precipitate and supernatant (2) add ammonium sulfate to the obtained supernatant to make the saturation to 40%, centrifuge after static treatment, and discard the precipitate; (3) utilize ultrafiltration membrane to carry out ultrafiltration to the resultant of step (2), concentrate to 20% to 30% of the original volume; (4) adding ethanol to the product of step (3), centrifuging after precipitation treatment, and taking the precipitate; (5) freeze-drying the product of step (4) to obtain the active polysaccharide Egg white; the raw material is at least one of Morchella, Polygonatum, and Snowdrop. The active polysaccharide protein obtained by the invention has high purity and high yield; compared with the prior art, the activity of the active polysaccharide protein obtained by the invention to inhibit oxygen free radicals, scavenge hydroxyl free radicals and inhibit tumor cells is greatly improved.

Description

technical field [0001] The invention specifically relates to fungi and plant active polysaccharide proteins such as hickory chick, polygonatum, snowdrop, etc., and a preparation method and application thereof. Background technique [0002] Carbohydrate-binding proteins are a class of binding proteins that have specific binding properties to various sugars. Most of them have hemagglutination activity, and some also have the effect of promoting cell mitosis. These properties are not significantly different from lectins, so some people call this type of protein a lectin, and some call it a receptor (receptors can be proteins, glycoproteins, glycolipids, or other compounds, so it should be said that they are multiple receptors one of the categories). For example, the galactose-specific binding protein on the mammalian liver cell membrane, Ashwell and other researchers also called it lectin and receptor in the same article, which are mainly named from different perspectives acc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K14/415C07K14/37A61K38/16A61P35/00A61P31/12A61P37/04
CPCA61K38/16C07K1/303C07K1/34C07K1/36C07K14/37C07K14/415
Inventor 秦小波鲍锦库牛蓓张国珍李圆圆徐莺宁华吴传芳张树梅吴霞
Owner 秦小波
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