Phosphorylated radix cyathulae polysaccharide, enema thereof and preparation method thereof

A technique of Achyranthes bidentata polysaccharide and Achyranthes bidentata, which is applied in the field of medicine, can solve the problems of not being able to directly kill canine parvovirus, weak direct killing effect of live virus, and weak antiviral effect, so as to prevent dogs from being infected with canine parvovirus Effect of virus, good antiviral effect, good therapeutic effect

Inactive Publication Date: 2017-10-20
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of canine parvovirus enteritis is mainly through systemic medication, mainly through the injection of antiviral preparations, such as ribavirin, virin and other western medicine preparations. The direct killing effect is weak
Therefore, resulting in a weaker antiviral effect
[0005] At present, there is no enema solution that can directly kill canine parvovirus and play a local antiviral role in the intestinal tract—phosphorylated Achyranthes bidentata polysaccharide enema solution

Method used

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  • Phosphorylated radix cyathulae polysaccharide, enema thereof and preparation method thereof
  • Phosphorylated radix cyathulae polysaccharide, enema thereof and preparation method thereof
  • Phosphorylated radix cyathulae polysaccharide, enema thereof and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0037] A method for preparing phosphorylated Achyranthes bidentata polysaccharide and its enema solution, the specific steps are as follows.

[0038] Step 1, extracting Achyranthes bidentata polysaccharide.

[0039] Step 1.1. Take 100g of Achyranthes bidentata, grind and crush it, add 1.0L of distilled water to boil for 2 hours, filter and collect the filtrate.

[0040] Step 1.2. Put the filtrate obtained in step 1.1 into a beaker, place it in a water bath at 60°C, and concentrate to 100 mL to obtain a polysaccharide extract of Achyranthes bidentata.

[0041] Step 1.3. Add 1 / 6 volume of chloroform and 1 / 18 volume of n-butanol to the Achyranthes bidentata polysaccharide extract obtained in step 1.2, shake for 3 minutes, and set the temperature at 2000 r / min Centrifuge for 10 min, and repeat this 3 times to obtain a centrifuge.

[0042] Step 1.4, add 6 times of absolute ethanol to the centrifuge in step 1.3, and let stand at 4°C for 12 hours. The resulting precipitate was was...

Embodiment 2

[0055] A method for preparing phosphorylated Achyranthes bidentata polysaccharide and its enema solution, the specific steps are as follows.

[0056] Step 1, extracting Achyranthes bidentata polysaccharide.

[0057] Step 1.1. Take 100g of Achyranthes bidentata, grind and crush it, add 1.5L of distilled water to boil for 3 hours, filter and collect the filtrate.

[0058] Step 1.2. Put the filtrate obtained in step 1.1 into a beaker, place it in a water bath at 60°C, and concentrate to 100 mL to obtain a polysaccharide extract of Achyranthes bidentata.

[0059] Step 1.3. Add 1 / 3 volume of chloroform and 1 / 17 volume of n-butanol to the Achyranthes bidentata polysaccharide extract obtained in step 1.2, shake for 4 minutes, and set the temperature at 2000 r / min Centrifuge for 12 min, and repeat this 4 times to obtain a centrifuge.

[0060] Step 1.4. Add 10 times of absolute ethanol to the centrifuge in step 1.3, and let stand at 4°C for 24 hours. The resulting precipitate was wa...

Embodiment 3

[0072] A method for preparing phosphorylated Achyranthes bidentata polysaccharide and its enema solution, the specific steps are as follows.

[0073] Step 1, extracting Achyranthes bidentata polysaccharide.

[0074] Step 1.1. Take 100g of Achyranthes bidentata, grind and crush it, add 1.2L of distilled water to boil for 2 hours, filter and collect the filtrate.

[0075] Step 1.2. Put the filtrate obtained in step 1.1 into a beaker, place it in a water bath at 60°C, and concentrate to 100 mL to obtain a polysaccharide extract of Achyranthes bidentata.

[0076] Step 1.3. Add 1 / 4 volume of chloroform and 1 / 16 volume of n-butanol to the Achyranthes bidentata polysaccharide extract obtained in step 1.2, shake for 5 minutes, and set the temperature at 2000 r / min Centrifuge for 15 min, and repeat this 5 times to obtain a centrifuge.

[0077] Step 1.4, add 8 times of absolute ethanol to the centrifuge in step 1.3, and let stand at 4°C for 48 hours. The resulting precipitate was was...

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Abstract

The invention provides phosphorylated radix cyathulae polysaccharide, enema thereof and a preparation method thereof. A preparation method of the enema of the phosphorylated radix cyathulae polysaccharide comprises the following steps: 1, extracting radix cyathulae polysaccharide; 2, preparing phosphorylated radix cyathulae polysaccharide; 3, preparing the enema of the phosphorylated radix cyathulae polysaccharide. The preparation method of the phosphorylated radix cyathulae polysaccharide comprises the following steps: adding the radix cyathulae polysaccharide into distilled water and nitric acid; heating and adding sodium phosphate and barium chloride; regulating a pH value; filtering; adding a filtrate into anhydrous sodium sulfate for configuration by oscillation to obtain a centrifugate; redissolving the centrifugate and dialyzing in a dialysis bag to obtain the phosphorylated radix cyathulae polysaccharide. The enema of the phosphorylated radix cyathulae polysaccharide is prepared from the phosphorylated radix cyathulae polysaccharide with mass concentration of 2 to 6 percent, sodium chloride with mass concentration of 0.7 to 2.1 percent, and alkalinity-acidity buffer liquid with mass concentration of 0.1 to 0.3 percent. The enema of the phosphorylated radix cyathulae polysaccharide prepared by the invention can directly kill canine parvovirus, and has a good treatment effect on puppies infected with the canine parvovirus; moreover, the survival rate of dogs infected with the canine parvovirus can be effectively improved.

Description

technical field [0001] The invention relates to an enema solution for treating dog diarrhea and a preparation method thereof, in particular to a phosphorylated Achyranthes bidentata polysaccharide, the enema solution and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] Canine parvovirus (Canine parvovirus, CPV) is a highly contagious virus, the pathogen of canine parvovirus infection (Canine parvovirus infection) mainly characterized by severe vomiting, diarrhea, hemorrhagic enteritis and suppurative myocarditis (Yan Wenqing et al., 2006) . Canine parvovirus is widely prevalent in my country. The main natural host of canine parvovirus is dogs. They are susceptible to dogs of different ages and genders, especially puppies are the most susceptible, and puppies before and after weaning are the most susceptible. Susceptible, in addition, canids such as foxes and minks are also the main source of infection for this disease....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61K9/08A61P31/20A61P1/12A61K33/14A61K33/00A61K33/06A61K31/19A61K31/7004A61K36/21
CPCC08B37/00A61K9/0031A61K9/08A61K31/19A61K31/7004A61K31/715A61K33/00A61K33/06A61K33/14A61K36/21C08B37/0003A61K2300/00
Inventor 封海波樊静
Owner SOUTHWEST UNIVERSITY
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