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Preparation method and application of Chinese yam polysaccharide-chromium complex

A technology of yam polysaccharide and chromium complex, which is applied in the field of medicine, can solve the problems such as unreported yam polysaccharide chromium, and achieves the effects of high development value, small adverse reactions, and improved immunity and anti-stress.

Inactive Publication Date: 2017-11-07
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

There have been a lot of physiological and biochemical experimental reports on polysaccharide chromium complexes at home and abroad, but there is no report on yam polysaccharide chromium

Method used

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  • Preparation method and application of Chinese yam polysaccharide-chromium complex
  • Preparation method and application of Chinese yam polysaccharide-chromium complex
  • Preparation method and application of Chinese yam polysaccharide-chromium complex

Examples

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Embodiment 1

[0033] 1 Purification of yam crude polysaccharide

[0034] (1) Preparation of enzyme solution: Accurately weigh 0.1g of trypsin, dissolve in 10mL of phosphate buffer (pH=7.0), and dissolve in a water bath at 37°C to obtain a 10mg / mL trypsin solution;

[0035] (2) Accurately weigh 5g of yam crude polysaccharide and dissolve it in 500 mL of pH=7 phosphate buffer, add 2ml of the prepared trypsin solution, place it in a water bath at 37°C and heat for 3 hours;

[0036](3) After the crude polysaccharide solution is enzymatically hydrolyzed, put it in boiling water to extinguish the enzyme for 10 minutes, cool at room temperature and centrifuge to discard the precipitate, add 2ml of Sevage (chloroform:n-butanol=4:1) solution to the supernatant, shake Shake the bed at a rate of 100r / min for 10min and let it stand for 30min, centrifuge at 3000r / min for 10min, take the supernatant, then add 3.5mL of Sevage reagent to the supernatant, repeat this process 4 times respectively; add 5 time...

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Abstract

The invention discloses a preparation method and application of Chinese yam polysaccharide-chromium complex. The Chinese yam polysaccharide-chromium complex is characterized in that the mass fraction of chromium in the Chinese yam polysaccharide-chromium complex is 5.1%, and the Chinese yam polysaccharide-chromium complex is prepared in a way that purified Chinese yam polysaccharide and chromium are subjected to self-assembling reaction under a certain condition. A reaction product is purified and separated, and an ultraviolet-visible spectroscopy method and an infrared spectroscopy method are subjected to coordination analysis to prove that the polysaccharide and Cr<3+> form a coordination compound. The mass ratio of a Cr element in the Chinese yam polysaccharide-chromium complex is measured as 5.1% by a flame atomic absorption spectroscopy method. An animal activity experiment indicates that the Chinese yam polysaccharide-chromium complex has a function of obviously lowering animal blood sugar and improving immunity. Therefore, the Chinese yam polysaccharide-chromium complex can be used for preparing medicines and health care food capable of resisting diabetes and immunomodulator.

Description

technical field [0001] The invention relates to a preparation method and application of a yam polysaccharide-chromium complex, belonging to the field of medicine. Background technique [0002] Diabetes is a disease that seriously threatens human life and health. The whole world is committed to the research and development of new diabetes drugs. It has always been the goal of drug developers to find effective, safe, and less adverse reactions. The active ingredient in blood sugar is an important pathway. As we all know, the occurrence of almost all diseases is related to the decline of autoimmunity, or the decline of the body's immunity due to disease. Polysaccharides have immune regulation, anti-tumor, hypoglycemic, anti-oxidation and other effects. Among them, the hypoglycemic and immunomodulatory effects are the common characteristics of polysaccharides and their main pharmacological activities. Chinese yam is a traditional Chinese medicine for both medicine and food, w...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K33/24A61P37/04A61P3/10A23L33/165A23L33/125A61K31/715
CPCA23V2002/00A61K31/715A61K33/24A23L33/125A23L33/165C08B37/00A61K2300/00A23V2200/324A23V2200/328
Inventor 李清禄邹凯能李宇翔应顺秋
Owner FUJIAN AGRI & FORESTRY UNIV
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