Bacillus for producing cellulase and laccase and application thereof in reconstituted tobacco

A technology of bacillus and tobacco flakes, which is applied in the field of bacillus to improve the quality of tobacco flake raw materials, can solve the problems of poor taste of paper-making tobacco flakes, and achieve the effects of ensuring the survival rate of bacteria, fast growth speed and easy cultivation

Active Publication Date: 2017-11-14
CHINA TOBACCO GUANGXI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For this reason, the technical problem to be solved by the present invention is to provide a kind of bacillus strain, to solve the problem that contains more harmful substance precursors in the raw material of tobacco sheet (mainly tobacco stem and tobacco dust) in the prior art, and then affects paper-making tobacco The problem of poor taste of flakes

Method used

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  • Bacillus for producing cellulase and laccase and application thereof in reconstituted tobacco
  • Bacillus for producing cellulase and laccase and application thereof in reconstituted tobacco
  • Bacillus for producing cellulase and laccase and application thereof in reconstituted tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Accurately weigh 6.0g of tobacco powder, mix with water at a ratio of 1:10 (w / v), stir and extract at 50°C for 2 hours, filter the obtained extract with suction, wash until the washing liquid is colorless, and dry to constant weight ,spare;

[0052] Preparation of liquid fermentation medium containing glucose, the formula is as follows: glucose group culture medium: glucose 3g / L, peptone 5g / L, tobacco leaf extract 3g / L, NaCl 5g / L, pH7.0;

[0053] The tobacco leaf extract added in the liquid fermentation medium is prepared according to the following method: take alcoholized tobacco leaves and deionized water, mix them according to the mass volume ratio (W:V) of 1:80, control the temperature at 180 Stir magnetically at -200°C for 1-1.5h, filter and sterilize with gauze to obtain the tobacco leaf extract;

[0054] The bacterial strain is inoculated in the test tube containing the liquid activation medium by 2% inoculum amount, and inoculated in the Erlenmeyer flask contai...

Embodiment 2

[0060] Accurately weigh 6.0g of tobacco powder, mix with water at a ratio of 1:10 (w / v), stir at 50°C for 2 hours for leaching, filter the obtained extract with suction, wash until the washing liquid is colorless, and dry to constant weight ,spare;

[0061] Prepare a liquid fermentation medium containing fructose, the formula is as follows: fructose group culture medium: fructose 3g / L, peptone 5g / L, tobacco leaf extract 3g / L, NaCl 5g / L, pH7.2;

[0062] The tobacco leaf extract added in the liquid fermentation medium is prepared according to the following method: take alcoholized tobacco leaves and deionized water, mix them according to the ratio of mass volume ratio (W:V) of 1:50, control the temperature at 180 Stir magnetically at -200°C for 1-1.5h, filter and sterilize with gauze to obtain the tobacco leaf extract;

[0063] The bacillus strain is inoculated in the test tube containing the liquid activation medium by 2% inoculum, and inoculated in the Erlenmeyer flask contai...

Embodiment 3

[0070] Accurately weigh 3.0g of tobacco powder and 3.0g of tobacco stems, mix them evenly, add water at a ratio of 1:10 (w / v) and mix evenly, stir at 50°C for 2 hours for leaching, filter the obtained extracts, and wash until the washing liquid Colorless, dried to constant weight, ready for use;

[0071] Preparation of liquid fermentation medium containing glucose, the formula is as follows: glucose group culture medium: glucose 3g / L, peptone 5g / L, tobacco leaf extract 3g / L, NaCl 5g / L, pH7.5;

[0072] The tobacco leaf extract added in the liquid fermentation medium is prepared according to the following method: take alcoholized tobacco leaves and deionized water, mix the two according to the ratio of mass to volume (W:V) of 1:100, and control the temperature at 180 Stir magnetically at -200°C for 1-1.5h, filter and sterilize with gauze to obtain the tobacco leaf extract;

[0073] Bacterial strain is inoculated in the test tube that contains described liquid activation medium ...

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Abstract

The invention belongs to the technical field of reconstituted tobacco production in combination with microbial fermentation, and particularly relates to a bacillus for producing cellulase and laccase, which is classified and named as Bacillus sp.GYC30 and is already preserved in China Center for Type Culture Collection (CCTCC) of Wuhan University and the preservation number of which is CCTCC M2016395. The bacillus strain which is screened out by the invention is obtained by screening from aged tobacco leaves, the growth state in a reconstituted tobacco material is good, the viability of the strain is strong, and moreover, the strain is high in activation rate, is easy to store, and has the advantages of strong tolerance to the environment, high growth speed, easiness in culturing and the like. When the strain disclosed by the invention is utilized for fermentation, cellulose and lignin in the reconstituted tobacco material can be effectively degraded, furthermore, the fermentation has the advantages of high strain growing ability, short fermentation period and little destruction to material in comparison with fungal fermentation, and therefore the invention has potential application value and a good development prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation combined with tobacco sheet production, specifically relates to a bacillus strain producing cellulase and laccase, and further discloses its use for improving the quality of tobacco sheet raw materials. Background technique [0002] Paper-Process Reconstituted Tobacco (Paper-Process Reconstituted Tobacco) is a product of tobacco resource recycling, mainly using some tobacco waste and leftovers in the tobacco manufacturing process, such as tobacco stems and tobacco leaves Fragments, tobacco powder, etc. are used as raw materials, and after extraction, concentration, separation, beating, refining, papermaking, drying, and flavoring processes, tobacco leaf thin slices close to natural tobacco leaves are made, used as cigarette fillers, and reused for An advanced tobacco production technology for cigarette production. [0003] Compared with tobacco sheets produced by other processes, p...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12N9/02C12N9/24C12N9/88A24B15/20A24B15/24A24B3/12C12R1/07
CPCA24B3/12A24B15/20A24B15/241C12N1/20C12N9/0061C12N9/2402C12N9/2437C12N9/88C12Y110/03002C12N1/205C12R2001/07
Inventor 龙章德张晓鸣刘鸿邹克兴李志华董其昌薛云孙建生于静洋李季刚
Owner CHINA TOBACCO GUANGXI IND
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