Method for simulating enzyme ageing of printing and drawing type silk relics in tombs

A technology for cultural relics and silk fabrics, which is applied in the biochemical treatment of enzymes/microorganisms, animal fibers, and biochemical fibers, etc. It can solve the problems of little research on the enzymatic aging method of cultural relics, and no research on the effect of the enzyme activity of the pigment layer, and achieve high activity. and lasting, the effect of promoting the aging process

Active Publication Date: 2017-11-17
ZHEJIANG SCI-TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the simulation research on the aging methods of printed and painted silk cultural relics mainly focuses on ultraviolet aging, light and humidity aging, heat aging, and microbial aging. In the study of fibers, there is no research on the pigment layer on the surface of the cultural relics, the protective layer on the surface of the pigment layer on the aging of enzymes, nor the influence of the pigment layer on the enzyme activity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] An enzymatic aging method for imitating printed and painted silk cultural relics in tombs, comprising the following steps:

[0022] (1) Heat the antique printed silk fabric in a solution A (made of 40% acetic acid, methanol, and deionized water in a volume ratio of 2:1.5:1) in a water bath at 60°C for 30 minutes, and then use a deionized Rinse with water twice, dry and then sterilize with ultraviolet light for 30 minutes, and cut into small pieces B.

[0023] (2) Prepare 800 parts by mass buffer solution (from 0.2mol / L Na 2 HPO 4 solution with 0.2mol / L NaH 2 PO 4 solution mixed), add 1 / 10000 parts by mass of streptavidin, and then add the enzyme promoter 8–((4–(2,4–dimethoxyphenyl)piperazin–1–yl)methyl )–5,7–dihydroxy–3–(4–hydroxyphenyl)–4H–benzopyran–4–one, the mass of which is twice that of streptomyces protease, was formulated as solution C.

[0024] (3) Completely immerse B in solution C and place it in a sterile incubator at 38°C for continuous aging. During t...

Embodiment 2

[0026] An enzymatic aging method for imitating printed and painted silk cultural relics in tombs, comprising the following steps:

[0027] (1) Heat the antique printed silk fabric in a solution A (made of 40% acetic acid, methanol, and deionized water in a volume ratio of 2:1:1) in a water bath at 60°C for 30 minutes, and then rinse with deionized water Rinse twice, dry and sterilize with ultraviolet light for 30 minutes, cut into small pieces B.

[0028] (2) Prepare 900 parts by mass buffer (from 0.2mol / L Na 2 HPO 4 solution with 0.2mol / L NaH 2 PO 4 solution mixed), add 1 / 10000 parts by mass of streptavidin, and then add the enzyme promoter 8–((4–(2,4–dimethoxyphenyl)piperazin–1–yl)methyl )–5,7–dihydroxy–3–(4–hydroxyphenyl)–4H–benzopyran–4–one, the quality of which is the same as that of streptomyces protease, was prepared as solution C.

[0029] (3) Completely immerse B in solution C, and place it in a sterile incubator at 38°C for continuous aging. During the aging per...

Embodiment 3

[0031] (1) Heat the antique printed silk fabric in a solution A (made from 40% acetic acid, methanol, and deionized water in a volume ratio of 2:1.5:1) in a 60°C water bath for 30 minutes, and then rinse with deionized water Rinse 3 times, dry and sterilize with ultraviolet light for 25 minutes, cut into small pieces B.

[0032] (2) Prepare 1000 parts by mass buffer solution (from 0.2mol / L Na 2 HPO 4 solution with 0.2mol / L NaH 2 PO 4 solution mixed), add 2 / 10000 parts by mass of streptavidin, and then add the enzyme promoter 8–((4–(2,4–dimethoxyphenyl)piperazin–1–yl)methyl )–5,7–dihydroxy–3–(4–hydroxyphenyl)–4H–benzopyran–4–one, the mass of which is twice that of streptomyces protease, was formulated as solution C.

[0033] (3) Immerse B completely in solution C and place it in a sterile incubator at 39°C for continuous aging. During the aging period, solution C is replaced every 48 hours. After 45 days, the enzymatic aging of simulated tomb printing and dyeing silk cultur...

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Abstract

The invention relates to the field of relic protection, and discloses a method for simulating enzyme ageing of printing and drawing type silk relics in tombs. The method comprises steps as follows: (1) antique printing and drawing silk fabric is treated with a solution A, washed with deionized water, subjected to ultraviolet sterilization treatment for 30-50 min after being air-dried and sheared into small pieces B; (2) 800-1,000 parts by mass of a buffer solution are prepared, 1/10000-2/10000 parts by mass of pronase from streptomyces gris are added, then, an enzyme promoter is added, and a solution C is prepared; (3) B is completely soaked in the solution C and continuously aged in a sterile incubator at 38+/-1 DEG C, and an enzyme ageing sample simulating the printing and drawing type silk relics in tombs can be obtained after 40-60 days. Through integration of multiple factors of enzyme for ageing of the relics, the enzyme ageing mode of the printing and drawing type silk relics in tombs can be effectively simulated, and a very important role is played on research of the enzyme ageing mechanism of the printing and drawing type silk relics and protection of relics.

Description

technical field [0001] The invention relates to the field of cultural relics protection, in particular to an enzymatic aging method for imitating printed and painted silk cultural relics in tombs. Background technique [0002] Printed and painted silk relics are the main carrier of Chinese traditional culture, and the extant parts are excavated from tombs. Because it is buried under the ground all the year round, the fiber will be degraded to varying degrees under the influence of temperature, humidity, soil acidity and alkalinity, bacteria, mold, etc., especially when it is in a humid environment, bacteria and mold will multiply in large numbers. Proteases such as pronase produced can cause devastating damage to cultural relics. Therefore, the protection of printed and painted silk cultural relics is imminent. Since the number of unearthed cultural relics is rare and they are very precious as precious physical witnesses of the history of civilization of the Chinese nation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M16/00D06M13/188D06M13/144D06M11/70D06M13/355D06M101/12
CPCD06M11/70D06M13/144D06M13/188D06M13/355D06M16/003D06M2101/12
Inventor 彭志勤池贺海万军民胡智文王秉丁文凤
Owner ZHEJIANG SCI-TECH UNIV
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