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A kind of method that improves tulip reproductive growth
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A technology of tulips and growth medium, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as heat intolerance, high sensitivity to temperature changes, and increased cultivation costs, so as to save costs and promote tissue growth. The effect of growth
Inactive Publication Date: 2019-06-11
界首市家丰家庭农场
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However, because tulip bulbs are expensive, cold-resistant but not heat-resistant, and highly sensitive to temperature changes, the development of tulip planting is limited; through tissue culture technology, it is difficult to replenish fresh culture medium due to the fixed culture medium, and different tissue growth stages The use of the same composition medium leads to low survival rate of tissue culture; and the use of different culture medium according to the tissue growth stage increases the cost of culture, and multiple transplants will also cause damage to tissue culture
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Embodiment 1
[0023] (1) Germination of bulbs: Place the harvested bulbs in an incubator at a temperature of 32°C and store them for 7 days; Days, the initial germination of bulbs;
[0024] (2) Sterilization treatment of bulbs: use metalaxyl wettable powder to prepare fungicide A, place the initially germinated tulip bulbs in fungicide A, and soak for 40 minutes; then, place the sterilized bulbs in a sterile In the box; as described in step (2), the composition and mass of fungicide A are: water 102kg, 25% metalaxyl wettable powder 0.1kg;
[0025] (3) Sterilization of the inoculation room: seal the inoculation room, use 95% ethanol to wipe the workbench, instrument surface and possible contact parts of the inoculation room, and turn on the ultraviolet light to irradiate the inoculation room for 4 hours; put tweezers, test tubes, inoculation knife, Erlenmeyer flasks and other inoculation tools and utensils are placed in an autoclave at a temperature of 121°C and autoclaved for 27 minutes; ...
Embodiment 2
[0040] (1) Germination of bulbs: Place the harvested bulbs in an incubator at a temperature of 34°C and store them for 7 days; Days, the initial germination of bulbs;
[0041] (2) Sterilization treatment of bulbs: use metalaxyl wettable powder to prepare fungicide A, place the initially germinated tulip bulbs in fungicide A, and soak for 40 minutes; then, place the sterilized bulbs in a sterile In the box; as described in step (2), the composition and mass of fungicide A are: water 108kg, 25% metalaxyl wettable powder 0.18kg;
[0042] (3) Sterilization of the inoculation room: seal the inoculation room, use 95% ethanol to wipe the workbench, instrument surface and possible contact parts of the inoculation room, and turn on the ultraviolet light to irradiate the inoculation room for 5 hours; put tweezers, test tubes, inoculation knife, Erlenmeyer flasks and other inoculation tools and utensils are placed in an autoclave at a temperature of 121°C and autoclaved for 28 minutes; ...
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Abstract
The invention discloses a method for improving reproduction and growth of tulips. The method is characterized by comprising the following steps: preparing different culture media at different phases; adding a plant cell division promoting component into a sprouting culture medium and adding a component capable of promoting the growth of roots, leaves and stems of tulip seedlings into a growth culture medium; fixing a tulip culture tissue on the solid-state sprouting culture medium through designing a tapered culture flask and a culture box; supplementing a fresh culture solution into the bottom of the tapered culture flask; conveying the culture solution into the culture box; supplementing and replacing the culture solution in the culture box through a liquid inlet hole and a liquid outlet hole; covering seedlings, which are obtained by tissue culture, with the solid-state sprouting culture medium before transplanting, so as to reutilize a waste culture medium and provide various nutritional and antibacterial components for soil transplanting of plants.
Description
technical field [0001] The invention belongs to the technical field of flower cultivation, and in particular relates to a method for improving the reproduction and growth of tulips. Background technique [0002] Tulips are widely planted all over the world. They are the national flowers of the Netherlands, New Zealand, Iran, Turkey, Turkmenistan and other countries. They have increasingly become a representative of fashion and internationalization. They are an important part of national foreign affairs activities, large-scale celebrations, Chinese and foreign flower shows, and high-end palaces . Tulips are favored by people for their colorful flower colors, long flowering period, nobility and elegance. The living environment increases the landscape of leisure and vacation. However, because tulip bulbs are expensive, cold-resistant but not heat-resistant, and highly sensitive to temperature changes, the development of tulip planting is limited; through tissue culture techno...
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