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Crop seedling-strengthening agent and use thereof

A seedling-strengthening agent and crop technology, applied in the field of agriculture, can solve problems such as no growth, increase economic value, etc.

Pending Publication Date: 2017-11-24
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These reports have carried out work on the fermentation formula, fermentation technology and metabolites of Inonotus obliquus, but did not involve liquid fermentation technology targeting extracellular β-glucan metabolites as indicators, nor did they use fermentation products as seedling strengthening agents. Research Report on Regulating Crop Growth and Improving Economic Value

Method used

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  • Crop seedling-strengthening agent and use thereof
  • Crop seedling-strengthening agent and use thereof
  • Crop seedling-strengthening agent and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of Inonotus obliquus Fruiting Body Extract

[0062] Wild Inonotus obliquus was purchased from the Changbai Mountain area of ​​Jilin Province. The wild Inonotus obliquus was dried at 60°C to constant weight, and then the fungus was crushed and sieved with 120 mesh (sieve hole size: 0.125mm) to obtain the obliquus obliquus. Bacteria powder. Accurately weigh 500 g of Inonotus obliquus powder, and add distilled water according to the ratio of volume to water of 1:40. Boil at 100°C for 2.5 hours, extract twice, combine the extracts, measure the polysaccharide content (the polysaccharide content is determined by the phenol-sulfuric acid method), dilute with water until the polysaccharide content is 10mg / mL, and it is biological bacteria solution No. 1.

Embodiment 2

[0064] Production of Submerged Fermentation Liquid of Inonotus obliquus

[0065] Strain preparation: the Inonotus obliquus bacterial strain (the present invention can also use other Inonotus obliquus bacterial strains) with the preservation number of CGMCC No.10297 is transferred twice with cPDA medium (comprehensive potato dextrose agar medium), each time Cultivate at 25°C for 10 days to obtain mother seed blocks. cPDA medium ( / L): potato 200.0g (leach juice), glucose 20.0g, peptone 0.5g, KH 2 PO 4 3.0g, MgSO 4 .7H 2O 1.5g, VB110mg, agar 14g, pH natural. Activated first-class medium: 87.5% fine sawdust (passed through a 20-mesh sieve, sieve size: 0.850mm), 20% wheat bran, 1% lime, 0.5% gypsum, 1% sugar, and 60% water content. Put the prepared activated primary seed medium into a 500mL triangular flask, each bottle contains 450g of wet material, seal it with a high-pressure filter film, sterilize at 121°C for 1.5h, cool down, insert 2 pieces of 1cm×1cm mother seed block ...

Embodiment 3

[0069] Effect of Soaking Seeds on Seed Germination

[0070] According to 1:25, 1:50, 1:100, 1:500, 1:1000, dilute biological bacteria solution No. 1 and biological bacteria solution No. 2 with water, and then use it with other seed soaking agents at the same time, and routine seed soaking management. Water was used as a blank control. The representative results of biological bacteria liquid No. 2 are as follows: figure 1 shown.

[0071] The results show that the crop seedling strengthening agent of the present invention diluted with water at 1:25, 1:50, 1:100, and 1:500 can all promote germination, especially the crop strong seedling agent diluted with water at 1:25, 1:50. The ability of seedling agent to promote germination is more significant.

[0072] This example also found that the germination period of the seeds was advanced by 2 days after soaking with the crop seedling strengthening agent of the present invention.

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Abstract

A crop seedling-strengthening agent and use thereof are disclosed, the crop seedling-strengthening agent comprises a polysaccharide-containing active ingredient which is effective to regulate the growth of a crop, the polysaccharide-containing active ingredient comprises a polysaccharide-containing active derived from Inonotus obliguus (Fr.) Pilat, the polysaccharide-containing active ingredient can improve at least one of the following traits of the crop: promoting plant growth, and making crop seedlings green in seedling bed periods green, strong and disease-resistant. The polysaccharide content of the active ingredient of the crop seedling-strengthening agent is 0.1-100 mg / mL. The polysaccharide-containing active ingredient derived from the Inonotus obliguus (Fr.) Pilat comprises an extract or a fermentation broth of the Inonotus obliguus (Fr.) Pilat. The crop seedling-strengthening agent may further comprise other active ingredients of edible and pharmaceutical fungi. The edible and pharmaceutical fungi are one or more selected from Ganoderma lucidum, shi-take mushrooms, hericium erinaceus, cordyceps sinensis, poria cocos, umbellate pore fungus, phellinus igniarius, fungus and Trametes versicolor. The crop seedling-strengthening agent is applied to the agricultural technical field.

Description

technical field [0001] The invention belongs to the technical field of agriculture and relates to a crop seedling strengthening agent, in particular to a crop seedling strengthening agent and its application. Background technique [0002] Inonotus obliguus (Fr.) Pilat is a rare medicinal fungus. Inonotus obliquus, also known as chaga and birch tears, is mainly distributed in Finland, Poland, Russia, Japan and my country. In my country, it is mainly distributed in Heilongjiang and Jilin. Inonotus obliquus rarely forms fruiting bodies in the wild environment, and the fruiting bodies grow slowly. It has medicinal value only when it grows on living birch trees for 10 to 15 years, and only one tree grows on every 20,000 birch trees. Inonotus obliquus. Therefore, wild resources of Inonotus obliquus are extremely rare. With the development of medicinal uses in recent years, the wild resources of Inonotus obliquus have suffered from predatory exploitation and are facing depletion...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01N63/04A01N65/00A01P21/00C12P19/08C12R1/645
CPCA01N43/16A01N63/30A01N65/00C12P19/08
Inventor 韩增华张丕奇戴肖东高娃孔祥辉马庆芳刘佳宁马银鹏陈贺王晗丛燕
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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