A detection method for the determination of monoamine neurotransmitters based on derivatization

Abstract

The invention discloses a detection method for determining monoamine neurotransmitters based on derivatization. According to the detection method, the monoamine neurotransmitters and derivative reagent n-butanal and the like generate derivative reaction, and an obtained derivatization product is analyzed and detected by an ultrahigh-phase liquid chromatogram-mass spectrum combined system; the monoamine neurotransmitters include dopamine, noradrenaline and 5-hydroxytryptamine. Derivatization reaction conditions of the method have the advantages of simpleness, gentleness, quickness and environmental friendliness; the method can process samples in batch and has the advantages of high analyzing speed, low analyzing method quantitative lower limit and good linear range, precise and accuracy; simultaneous quantitative analysis on the dopamine, the noradrenaline and the 5-hydroxytryptamine in trace rat brain microdialysate is achieved.

Description

technical field [0001] The present invention relates to the technical field of analytical chemistry, in particular to a detection method for the determination of various monoamine neurotransmitters based on a derivatization method, in particular to a method using n-butyraldehyde as a derivatization reagent, combined with microdialysis technology and ultra-high performance liquid Analytical Method for the Simultaneous Detection of Multiple Neurotransmitters in Rat Brain Microdialysates by Phase Chromatography-Mass Spectrometry. Background technique [0002] The central nervous system (central nervous system, CNS) is the structure where neurons are concentrated in the nervous system, and it is the command center that controls the consciousness, psychology, thinking activities and specific behaviors of vertebrates. An important class of neurons is monoaminergic neurons, whose terminals release monoamine neurotransmitters such as norepinephrine (NE), dopamine (DA) and 5-hydroxyt...

AI Technical Summary

Problems solved by technology

However, phthalaldehyde derivatives have disadvantages such as instability, poor repeatability, and long derivation process time, which limit their wide use to a certain extent; benzoyl chloride and 4'-carbonyl chloride-rhodamine are easily hydrolyzed, and The skin, eyes and respiratory tract are irritating; deuterated acetaldehyde is expensive and not easy to obtain. Careless operation during the reaction may produce highly toxic hydrogen cyanide, and the reproducibility is poor

Therefore, in view of the low sample volume of microdialysis samples in the prior art, the low concentration of most neurotransmitters and other difficulties, as well as the poor reproducibility, low sensitivity, serious matrix interference, complex derivatization process, and the need to use Expensive and heavily polluting derivatization reagents, insufficient safety and environmental protection, etc., to develop a derivatization reaction with mild conditions, simple, fast, efficient, safe, environmentally friendly, good reproducibility, high sensitivity and can simultaneously detect a variety of monoamine neurons The analysis method of transmitter will be a research of great practical significance

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