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Persimmon leaf polyphenol with skin-whitening and sunscreening activity and preparation method and application thereof

A persimmon leaf, whitening technology, applied in the field of persimmon leaf polyphenols and its preparation, can solve the problems such as the extraction method of persimmon leaf residue or the patent application is not retrieved, and achieve the effect of inhibiting the formation of melanin, high content, and scavenging free radicals

Inactive Publication Date: 2017-12-15
FOSHAN SANSHUI DISTRICT JIAHUA CHEM RES INST GENERAL PARTNERSHIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on persimmon leaves is mainly focused on the efficacy of the ingredients, and there is no corresponding record on the extraction method or patent application of persimmon leaf residue

Method used

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  • Persimmon leaf polyphenol with skin-whitening and sunscreening activity and preparation method and application thereof
  • Persimmon leaf polyphenol with skin-whitening and sunscreening activity and preparation method and application thereof
  • Persimmon leaf polyphenol with skin-whitening and sunscreening activity and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing persimmon leaf polyphenols with whitening and sunscreen activity, comprising the following preparation steps:

[0025] (1) drying the persimmon leaf residue after alcohol extraction at 50° C., and passing through a 70-mesh sieve to obtain the persimmon leaf residue powder;

[0026] (2) Add 3 times the weight of distilled water to the persimmon leaf residue powder, adjust the pH to 5.5, add 0.5% protease, mix well, place in a constant temperature water bath at 60°C for 1 hour, add 25 times of absolute ethanol and let it stand for 8 hours , sonicated for 20 min, centrifuged at 3000r / min to obtain a supernatant, repeated twice, combined with the supernatant, and recovering the organic extractant under reduced pressure to obtain a concentrated solution;

[0027] (3) Extract the concentrated solution with methanol, let the mixed extract stand for 10 hours, discard the organic extractant layer after layering, concentrate under reduced pressure, and vacu...

Embodiment 2

[0029] A method for preparing persimmon leaf polyphenols with whitening and sunscreen activity, comprising the following preparation steps:

[0030] (1) drying the persimmon leaf residue after alcohol extraction at 40°C, and passing through a 60-mesh sieve to obtain the persimmon leaf residue powder;

[0031] (2) Add 3 times the weight of distilled water to the persimmon leaf residue powder, adjust the pH to 5.5, add 0.1% protease, mix thoroughly, place in a constant temperature water bath at 60°C for 1 hour, add 20 times of absolute ethanol and let stand for 8 hours , sonicate for 20 min, centrifuge at 3000r / min to obtain a supernatant, repeat twice, combine the supernatants, recover absolute ethanol under reduced pressure, and obtain a concentrated solution;

[0032] (3) Extract the concentrated solution with methanol, let the mixed extract stand for 10 hours, discard the methanol after layering, concentrate under reduced pressure, and vacuum freeze-dry to obtain persimmon l...

Embodiment 3

[0034] A method for preparing persimmon leaf polyphenols with whitening and sunscreen activity, comprising the following preparation steps:

[0035] (1) drying the persimmon leaf residue after alcohol extraction at 60° C., and passing through an 80-mesh sieve to obtain the persimmon leaf residue powder;

[0036] (2) Add 3 times the weight of distilled water to the persimmon leaf residue powder, adjust the pH to 5.5, add 1% protease, mix well, place in a constant temperature water bath at 60°C for 1 hour, add 30 times of absolute ethanol and let it stand for 8 hours , sonicate for 20 min, centrifuge at 3000r / min to obtain a supernatant, repeat twice, combine the supernatants, recover absolute ethanol under reduced pressure, and obtain a concentrated solution;

[0037] (3) Extract the concentrated solution with methanol, let the mixed extract stand for 10 hours, discard the methanol after layering, concentrate under reduced pressure, and vacuum freeze-dry to obtain persimmon lea...

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Abstract

The invention discloses a persimmon leaf polyphenol with whitening and sunscreen activity and its preparation method and application. Persimmon leaf residue powder; (2) Add 3 times the weight of distilled water to the persimmon leaf residue powder, adjust the pH to 5.5, add protease, mix thoroughly, place in a constant temperature water bath at 60°C for 1 hour, add an organic extractant and let it stand 8h, sonicate for 20min, and centrifuge at 3000r / min to obtain a supernatant, repeat twice, combine the supernatant, recover the organic extractant under reduced pressure, and obtain a concentrated solution; (3) extract the concentrated solution with an organic extractant, The mixed extract was left to stand for 10 hours, and after the layers were separated, the organic extract layer was discarded, concentrated under reduced pressure, and vacuum freeze-dried to obtain persimmon leaf polyphenols. The invention can scavenge free radicals, has anti-oxidation effect, can well absorb ultraviolet rays, and inhibits the formation of melanin, so as to achieve the effect of whitening and sun protection.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a persimmon leaf polyphenol with whitening and sunscreen activity, a preparation method and application thereof. Background technique [0002] As the environmental pollution becomes more and more serious, the destruction of the atmospheric ozone layer is also more and more serious, resulting in the intensity of ultraviolet rays in the sun becoming stronger, and the damage of ultraviolet rays to the skin of people under the light is becoming more and more obvious. Melanin-promoting factors are secreted from glial cells in the epidermis of the skin when the skin is exposed to ultraviolet rays. This factor stimulates melanocytes to synthesize melanin, which darkens the epidermis of the skin due to the release of this melanin to the skin surface. [0003] Persimmon leaves are the fresh or dried leaves of Persimmon persimmon, a plant of the genus Persimmon. As a medicine, it was first record...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/02A61Q17/04A61Q19/08A61Q19/00A61K8/98
CPCA61K8/97A61K8/987A61K2800/10A61K2800/84A61Q17/04A61Q19/00A61Q19/02A61Q19/08
Inventor 不公告发明人
Owner FOSHAN SANSHUI DISTRICT JIAHUA CHEM RES INST GENERAL PARTNERSHIP
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