Method for detecting drug sensitivity of in-vitro blood culture anti-tumor medicine

An in vitro culture and anti-tumor drug technology, applied in the field of biomedicine, can solve problems such as patient pain, patient trauma, and unfavorable patient health recovery, and achieve the effect of delaying treatment time, simple source, and efficient anti-tumor drug sensitivity detection

Inactive Publication Date: 2017-12-22
四川精准医学检验有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. Whether the tissue samples obtained through biopsy or surgery will cause a certain degree of trauma to the patient, which will not only cause unavoidable pain to the patient, but also be detrimental to the patient's health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] For 75 patients with lung cancer and 25 patients with non-malignant tumors, 7.5mL of peripheral blood were collected, stored and transported at 4th degree. Tumor platelets in blood samples were enriched and separated by immunomagnetic bead technology. Centrifuge for 15 minutes, 2000 rpm. Dilute and wash with PBS. Then continue to wash with basal medium. Add the prepared collagen 3D culture solution to the washed cells, transfer them all to culture bottles, and incubate overnight in an incubator. The conditions when carrying out three-dimensional culture are as follows: O 2 Content at 1%, CO 2 The content is 5%, the pressure is 2psi, the temperature is 37°C, and the humidity is 95%. The next day, the drug to be tested was added to each well, and three parallel wells were set up for each drug, and a control group without drug addition was set up. Put the 96-well plate in a humidity greater than 95%, 37°C, 5% CO 2 , and incubate for 4 days in a cell culture incubato...

Embodiment 2

[0035] Selected 120 cases of ovarian tumor patients, including 78 cases of ovarian cancer group. Ovarian borderline tumor group included 42 cases, aged 24 to 72 years, with a median age of 51 years. Ovarian benign tumor group consisted of 50 cases, aged 18 to 71 years, with a median age of 49 years. A total of 60 healthy females who underwent physical examination at the same time were selected as the control group, aged 22 to 78 years, with a median age of 50 years. Take 7.5mL of venous blood from the patient on an empty stomach in the morning (without radiotherapy, chemotherapy or other treatment before taking the sample), put it into an anticoagulant tube containing EDTA-K2 and mix well, and use XE-2000 whole blood cell automatic detection and analysis within 2 hours after blood collection The instrument detects the platelet count. The conditions when carrying out three-dimensional culture are as follows: O 2 Content at 1%, CO 2 The content is 5%, the pressure is 2psi, t...

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Abstract

The invention discloses a method for detecting drug sensitivity of an in-vitro blood culture anti-tumor medicine, belongs to the technical field of biological medicines, and aims to solve the problems that conventional drug sensitivity detection needs biopsies or tissue samples obtained from operations and cannot be implemented at an early stage of a tumor. The method comprises the following steps: a) collecting peripheral blood; b) separating tumor thrombocyte, circulating tumor cells and tumor stem cells in the peripheral blood; c) performing three-dimensional culture on the separated tumor thrombocyte, the circulating tumor cells and the tumor stem cells in collagen; d) adding a to-be-detected medicine, and performing incubation in a cell culture tank; e) adopting an ATP-TCA (adenosine triphosphate-tumor chemosensitivity assay) or MTT (methyl thiazolyl tetrazolium) method to perform morphology and growth situation identification, thereby obtaining drug sensitivity information of an anti-tumor medicine. According to the method, drug sensitivity is tested through extraction of CTC (circulating tumor cell), CSC (cancer stem cell) and TEPs (tumor-educated platelets) from whole blood, the medicine is adopted as a target substance for blood biopsies of tumor patients and drug sensitivity studies, and the method is a novel idea for early-stage tumor screening and drug sensitivity testing and has wide application prospects.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for detecting drug susceptibility of antitumor drugs cultured in vitro. Background technique [0002] The current incidence of cancer ranks first in the world. How to choose drugs for cancer patients and carry out targeted and precise treatment is the current concern of clinical medical staff. [0003] In vitro drug sensitivity test of antineoplastic drugs can advise medical staff which drug or drugs to choose to accurately treat an individual tumor patient, so as to achieve precise treatment of tumor chemotherapy drugs and avoid serious adverse reactions caused by chemotherapy drugs. At present, the commonly used samples for drug susceptibility tests of antineoplastic drugs are tissue samples obtained by biopsy or surgery. [0004] The existing detection methods for antineoplastic drug susceptibility have the following problems: [0005] 1. Whether it is a tissue...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5011G01N33/5026G01N33/5044
Inventor 吴英王毅
Owner 四川精准医学检验有限责任公司
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