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Quality control method of DNA (Deoxyribonucleic Acid) ploidy analysis equipment

A quality control method and analysis equipment technology, which is applied to the analysis of materials, material analysis through optical means, and measurement devices, etc., can solve the problems of inaccurate analysis results and achieve the effect of accurate and error-free analysis of samples

Inactive Publication Date: 2017-12-29
黑龙江然得基尔医学科技发展有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a quality control method for DNA ploidy analysis equipment in order to solve the problem of inaccurate analysis results caused by equipment or sample problems in the prior art

Method used

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  • Quality control method of DNA (Deoxyribonucleic Acid) ploidy analysis equipment
  • Quality control method of DNA (Deoxyribonucleic Acid) ploidy analysis equipment
  • Quality control method of DNA (Deoxyribonucleic Acid) ploidy analysis equipment

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specific Embodiment approach 1

[0032] Specific embodiment 1: A method for quality control of DNA ploidy analysis equipment includes the following steps:

[0033] Step 1: Adjust the light source in the microscope by analyzing the pictures under the microscope field of view to make the light intensity and light uniformity meet the requirements;

[0034] If T low ≤p max ≤T high Then the light intensity meets the requirements, where T low ,T high Respectively represent the minimum and maximum of the light source intensity that meets the requirements, T low ,T high After many experiments and theoretical explorations, it can make the equipment operate normally, keep the image from being too dark or too bright, affecting the analysis of cells, and the result is within the allowable error range, and will not lead to misdiagnosis and missed diagnosis, p max Is p(p k ), p(p k ) Is the mean value of the number of pixels corresponding to the gray level in the smoothing window;

[0035] If both meet And The light is even...

specific Embodiment approach 2

[0045] Specific embodiment two: this embodiment is different from specific embodiment one in that the specific process of judging the intensity of light and the uniformity of light by analyzing the pictures under the microscope field in the first step is:

[0046] Step 1: Place the sample sheet on the platform and adjust the platform up and down to make a clear cell image under the camera's field of view (the collected cell image has a clear outline, no blur, and no blur, that is, the focus of the microscope objective lens coincides with the edge of the cell screen) ;

[0047] Step one and two: Grab the picture with the camera. The black and white picture of the cell under the microscope is represented by A0, A0(i,j) represents the gray level at the image position (i,j), 1≤i≤M,1≤j≤ N; M is the number of pixels in the horizontal direction in the captured image, and N is the number of pixels in the vertical direction in the captured image;

[0048] Step 13: Calculate the light intensi...

specific Embodiment approach 3

[0066] Specific embodiment three: This embodiment is different from specific embodiment one or two in that the specific process of determining the impurities in the optical path by analyzing the picture of the blank film under the mirror in the second step is:

[0067] Step 2: Place a blank film and adjust the platform up and down to make a clear cell image under the camera's field of view;

[0068] Step 2: Use the camera to capture the black and white picture under the microscope, denoted by A, A(i,j) represents the gray value at the image position (i,j), 1≤i≤M, 1≤j≤N;

[0069] Step two and three: control the electric platform to move a field of view, and use the camera to collect picture B;

[0070] Step two and four: Divide images A and B, and locate the impurity position; the impurity here may come from the light path or the blank film.

[0071] Step two and five: locate the position of impurities on A and B. For impurities at the same position on A and B, calculate the characterist...

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Abstract

The invention relates to a quality control method of DNA (Deoxyribonucleic Acid) ploidy analysis equipment. By adopting the quality control method of the DNA ploidy analysis equipment, the problem in the prior art that an analysis result is not accurate due to the problems of equipment or a sample is solved. The quality control method provided by the invention comprises the following steps: step 1, enabling illumination intensity and illumination uniformity to meet requirements; step 2: determining impurities in a light path through analyzing a picture of a blank slide under a microscope and carrying out impurity removal treatment; step 3: judging whether the equipment is at a normal working state or not; step 4: judging whether a sample slide of a patient is dyed uniformly or not; step 5: after determining a calibration zero point used in a microscope focusing process by utilizing a three-point fixed-point focusing manner, calculating positions of cells, relative to the calibration zero point, on a microscope slide in each view field through counting up movement distances of a control focusing platform; drawing a three-dimensional line graph; adjusting a microscope platform according to the three-dimensional line graph. The quality control method of the DNA ploidy analysis equipment is used for the field of instrument analysis.

Description

Technical field [0001] The invention relates to a quality control method for DNA ploidy analysis equipment. Background technique [0002] In recent years, malignant tumors are at a high incidence in my country. According to statistics, about 7 people are diagnosed as malignant tumors every minute in my country. This has become the most important cause of the fatality rate in our country and has aroused great attention from governments at all levels. Take Cervical Cancer as an example. It is the third largest female malignant tumor in the world and the second most common malignant tumor in Chinese women. According to estimates by the World Health Organization (WHO), there are more than 470,000 new cases of cervical cancer each year in the world, and China accounts for 28%. Early diagnosis and early treatment is an effective way to deal with the high incidence of cancer. Pathologists collect human exfoliated cells, including cervical exfoliated cells, puncture fluid, sputum, uri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/17
CPCG01N21/17
Inventor 陈兴路
Owner 黑龙江然得基尔医学科技发展有限公司
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