Method for regulating molecular weight of schizophyllan as fermentation product of schizophyllum commune franch

A technology for schizophyllan and fermentation products, which is applied in the field of regulating the molecular weight of schizophyllan fermentation products of Schizophyllum, can solve problems such as product inactivation, and achieve the effects of simplifying processes, reducing production costs, and simplifying preparation steps

Active Publication Date: 2018-01-09
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, the chemical degradation method of schizophyllan is mainly acid degradation, and the physical degradation method is mainly ultrasonic degradation, and the biological enzymatic hydrolysis method is a better degradation method, but it is difficult to find a specific enzyme to meet the requirements of schizophyllan. The need for polysaccharide degradation and modification, on the one hand, the enzyme must be β-1,3-endoglucanase, on the other hand, many enzymes will degrade polysaccharides below 50kDa (the product loses activity) and cannot meet the requirements

Method used

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  • Method for regulating molecular weight of schizophyllan as fermentation product of schizophyllum commune franch
  • Method for regulating molecular weight of schizophyllan as fermentation product of schizophyllum commune franch
  • Method for regulating molecular weight of schizophyllan as fermentation product of schizophyllum commune franch

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The slant strain of Schizophyllum commune Fr. was purchased from the Guangdong Province Microbial Culture Collection in October 2014, and the deposit number of the strain is GIM5.43. The seed activation medium adopts PDA medium;

[0039] PDA slant medium composition: peel the potato, cut into small pieces, add water and boil for 30 minutes, filter with double gauze, add sugar and agar to the filtered juice, boil to dissolve the agar, make up the amount of water to 1000mL, and 0.1MPa after aliquoting Bacteria for 20 minutes; per 1000 mL of water, the amount of potato is 200g, the amount of sugar is 20g, the amount of peptone is 5g, and the amount of agar is 20g.

[0040] Seed activation: take the pre-prepared and cooled test tube slope, use a sampling loop in the ultra-clean table to take the hyphae from the purchased test tube slope strain, and then streak the inoculation on the newly prepared test tube slope. Then put the inoculated slope into an incubator at 27°C for 6 da...

Embodiment 2

[0054] The slant strain of Schizophyllum commune Fr. was purchased from the Guangdong Province Microbial Culture Collection in October 2014, and the deposit number of the strain is GIM5.43. The seed activation medium adopts PDA medium;

[0055] PDA slant medium composition: peel the potato, cut into small pieces, add water and boil for 35 minutes, filter with double gauze, add sugar and agar to the filtered juice, boil to dissolve the agar, make up the amount of water to 1000mL, and 0.1MPa after aliquoting Bacteria in 25 minutes; per 1000mL of water, the amount of potato is 200g, the amount of sugar is 20g, the amount of peptone is 5g, and the amount of agar is 20g.

[0056] Seed activation: take the pre-prepared and cooled test tube slope, use a sampling loop in the ultra-clean table to take the hyphae from the purchased test tube slope strain, and then streak the inoculation on the newly prepared test tube slope. Then put the inoculated slope into an incubator at 26°C for 7 days...

Embodiment 3

[0069] The slant strain of Schizophyllum commune Fr. was purchased from the Guangdong Province Microbial Culture Collection in October 2014, and the deposit number of the strain is GIM5.43. The seed activation medium adopts PDA medium;

[0070] PDA slant culture medium composition: peel the potato, cut into small pieces, add water and boil for 40 minutes, filter with double gauze, add sugar and agar to the filtered juice, boil to dissolve the agar, make up the amount of water to 1000mL, and 0.1MPa after aliquoting Bacteria for 30 minutes; per 1000mL of water, the amount of potato is 200g, the amount of white sugar is 20g, the amount of peptone is 5g, and the amount of agar is 20g.

[0071] Seed activation: Take the pre-prepared and cooled test tube slope, use a sampling ring in the ultra-clean table to take the hyphae from the purchased test tube slope strain, and then inoculate the newly prepared test tube slope with a streak. Then put the inoculated slope into an incubator at 28...

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Abstract

The invention discloses a method for regulating the molecular weight of schizophyllan as a fermentation product of schizophyllum commune franch. The method comprises the following steps: taking slopestrains of schizophyllum commune franch, and carrying out slope inoculation and culture by adopting a PDA culture medium test tube, thereby preparing fermentation seed liquid; inoculating the seed culture liquid into a fermentation tank for fermentation, wherein the initial stage of fermentation is 0-36 hours, and the fermentation conditions are controlled as follows: the stirring rotation speed is 100-400r / min, the ventilation volume is 0.20-0.8vvm, and the temperature is 26-28 DEG C; the middle stage of fermentation is 37-84 hours, and the later stage of fermentation is 85-96 hours; and respectively controlling the fermentation conditions, discharging after the fermentation is ended, and removing mycelia; concentrating, precipitating, filtering to obtain refined schizophyllan. Accordingto the method disclosed by the invention, polysaccharides are degraded by utilizing enzyme self-secreted by the thalli, so that not only is the procedure of degrading after separating and purifying crude polysaccharides with larger molecular weight and poorer solubility and obtained by fermentation in the traditional method greatly simplified, but also the product yield is increased and the production cost is reduced.

Description

Technical field [0001] The present invention relates to Schizophyllan, in particular to a method for regulating the molecular weight of Schizophyllan fermentation product of Schizophyllum schizophylla -D-glucanase enzymatically hydrolyze polysaccharides to adjust the molecular weight of the fermentation product Schizophyllan. Background technique [0002] Schizophyllan (SPG) is a water-soluble polysaccharide fermented by Schizophyllum spp. It has a variety of physiological activities such as inhibiting tumors, antibacterial and anti-inflammatory, anti-radiation, and improving immunity. However, the schizophyllan obtained by fermentation has a large molecular weight (generally more than 4000kDa), is difficult to re-dissolve, and is not conducive to bioabsorption, which greatly limits its application. It needs to be degraded and the molecular weight is moderately reduced, but excessive degradation will cause Cause the loss of its biological activity. [0003] At present, in the pri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C12R1/645
Inventor 郑必胜邓娜吴丽华周林周立武刘瑞海
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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