Method for regulating molecular weight of schizophyllan as fermentation product of schizophyllum commune franch
A technology for schizophyllan and fermentation products, which is applied in the field of regulating the molecular weight of schizophyllan fermentation products of Schizophyllum, can solve problems such as product inactivation, and achieve the effects of simplifying processes, reducing production costs, and simplifying preparation steps
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Embodiment 1
[0038] The slant strain of Schizophyllum commune Fr. was purchased from the Guangdong Province Microbial Culture Collection in October 2014, and the deposit number of the strain is GIM5.43. The seed activation medium adopts PDA medium;
[0039] PDA slant medium composition: peel the potato, cut into small pieces, add water and boil for 30 minutes, filter with double gauze, add sugar and agar to the filtered juice, boil to dissolve the agar, make up the amount of water to 1000mL, and 0.1MPa after aliquoting Bacteria for 20 minutes; per 1000 mL of water, the amount of potato is 200g, the amount of sugar is 20g, the amount of peptone is 5g, and the amount of agar is 20g.
[0040] Seed activation: take the pre-prepared and cooled test tube slope, use a sampling loop in the ultra-clean table to take the hyphae from the purchased test tube slope strain, and then streak the inoculation on the newly prepared test tube slope. Then put the inoculated slope into an incubator at 27°C for 6 da...
Embodiment 2
[0054] The slant strain of Schizophyllum commune Fr. was purchased from the Guangdong Province Microbial Culture Collection in October 2014, and the deposit number of the strain is GIM5.43. The seed activation medium adopts PDA medium;
[0055] PDA slant medium composition: peel the potato, cut into small pieces, add water and boil for 35 minutes, filter with double gauze, add sugar and agar to the filtered juice, boil to dissolve the agar, make up the amount of water to 1000mL, and 0.1MPa after aliquoting Bacteria in 25 minutes; per 1000mL of water, the amount of potato is 200g, the amount of sugar is 20g, the amount of peptone is 5g, and the amount of agar is 20g.
[0056] Seed activation: take the pre-prepared and cooled test tube slope, use a sampling loop in the ultra-clean table to take the hyphae from the purchased test tube slope strain, and then streak the inoculation on the newly prepared test tube slope. Then put the inoculated slope into an incubator at 26°C for 7 days...
Embodiment 3
[0069] The slant strain of Schizophyllum commune Fr. was purchased from the Guangdong Province Microbial Culture Collection in October 2014, and the deposit number of the strain is GIM5.43. The seed activation medium adopts PDA medium;
[0070] PDA slant culture medium composition: peel the potato, cut into small pieces, add water and boil for 40 minutes, filter with double gauze, add sugar and agar to the filtered juice, boil to dissolve the agar, make up the amount of water to 1000mL, and 0.1MPa after aliquoting Bacteria for 30 minutes; per 1000mL of water, the amount of potato is 200g, the amount of white sugar is 20g, the amount of peptone is 5g, and the amount of agar is 20g.
[0071] Seed activation: Take the pre-prepared and cooled test tube slope, use a sampling ring in the ultra-clean table to take the hyphae from the purchased test tube slope strain, and then inoculate the newly prepared test tube slope with a streak. Then put the inoculated slope into an incubator at 28...
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