An all-in-one device for rapid Raman measurement and laser ejection sorting of single cells
A single-cell, laser technology, applied in Raman scattering, material excitation analysis, sorting, etc., can solve the problems of exogenous DNA contamination, limiting sorting flux and efficiency, etc., to avoid external exposure, improve efficiency, reduce effect of possibility
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Embodiment 1
[0048] Example 1: Production and effect of laser ejection substrates.
[0049] The substrate of the laser ejection substrate uses a light transparent material including, but not limited to, silicate glass, quartz glass, calcium fluoride glass; the bubble plating film material is a transparent metal composite oxide film material, including but not limited to ITO, FTO, AZO , A plating film is formed on the surface of the base material in the surface of the base material, the thickness of the metal composite oxide film is not less than 70% by deposition of spraying, evaporation, sputtering and other deposition. Figure 1 The light-transmitted comparison of the quartz glass substrate is shown, and the light-transmitting comparison of the ITO film, 1a is a quartz glass substrate, 1B is a laser ejection substrate plated with an ITO film). The laser ejection substrate (1b) plated with a plated film compared to the substrate (1a), the light transmittance is almost no different, and the sin...
Embodiment 2
[0050] Example 2: Sample chip, production and integrated device combination of the receiving chip
[0051] Figure 4 A single-cell laser echo sorting integrated device is schematically illustrated, and the single cell laser echo sorting sorting integrated device includes a laser ejection substrate 1, a point-like chip 2, and a receiving chip 3.
[0052] The point-like chip 2 employs a transparent soft polymer material including, but not limited to, PDMS, silicone rubber, PVC, thickness is made of 0.1-2 mm, and the chip contains an array microchannel 2-1, and the microchannel diameter is 0.5-2.5mm. .
[0053] The receiving chip 3 employs a transparent material including, but not limited to, one or several combinations of silicate glass, PDMS, PMMA, and the chip contains a certain depth of array micro-well 3-1, and the micro-well size is 1-5mm. Its position corresponds to the micropolor position in the above-described point-like chip.
[0054] The monocyte laser echo sorting integra...
Embodiment 3
[0055] Example 3: Single-cell fast Raman measurement and sorting by integrated devices
[0056] Taking marine blue bacteria, a single cell laser echo sorting integrated device is used to perform single-cell fast Raman measurement and sorting method, including the following procedure:
[0057] The spotty chip 2 is brought into laser ejection substrate 1 plating film 1-2 front, laboratory pure culture marine blue bacteria, straw over water after centrifugation and pure water, resuspended in water, take 0.5 μL of cell suspension drip Dotted in the point, the room temperature is dry. The receiving chip 3 is then bonded to the point-like chip 2, and the micrometer 3-1 is integrally formed with the point-like hole one by one. The above single cell laser echo sorting integrated device is placed on the stage of the front-grade co-equivalent, the laser ejection substrate 1 is on, the receiving chip 3 is under, passed through the 50X microscope objective, passed through the laser ejection s...
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