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A kit for rapid microscopic examination diagnosis of swine eperythrozoonosis and a preparing method thereof

A technology of porcine epierythrocytes and diagnostic kits, which is applied in the field of veterinary inspection, can solve the problems of many factors affecting detection operations and high technical requirements, and achieve the effects of simple detection methods, simple preparation methods, and low cost

Inactive Publication Date: 2018-01-16
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The PCR diagnostic method has strong specificity, but the technical requirements are relatively high, and there are many factors affecting the detection operation

Method used

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  • A kit for rapid microscopic examination diagnosis of swine eperythrozoonosis and a preparing method thereof
  • A kit for rapid microscopic examination diagnosis of swine eperythrozoonosis and a preparing method thereof
  • A kit for rapid microscopic examination diagnosis of swine eperythrozoonosis and a preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the preparation of porcine eperythrozoonosis rapid microscopic examination diagnostic kit

[0038] 1. Washing of utensils and instruments for rapid mirror kits:

[0039] 1.1 Soak the required glassware in a glassware tank filled with strong acid oxidation cleaning agent overnight;

[0040] 1.2 Take out the glassware soaked in strong acid oxidizing cleaning agent overnight, wash it with hot soapy liquid, rinse it with tap water 5 times, soak it in distilled water for 1 hour, take out the glassware and let it dry naturally.

[0041] 1.3 Put the glassware into a dry disinfection box at 138°C for dry heat disinfection for 1 hour, take it out after cooling and put it in a constant temperature drying box at 35°C for later use.

[0042] 1.4 Washing of dropper tip and plastic reagent bottle: put the dropper tip and plastic bottle into clean water and soak for 30 minutes; wash the dropper tip and plastic bottle soaked in clean water with hot soapy liquid, and rins...

Embodiment 2

[0058] Embodiment 2: Eperythrozoonis porcine rapid microscopic examination diagnostic kit operation:

[0059] 1. Collection of blood samples

[0060] 1.1 Blood collection from the ear vein: select a medium-sized and protruding vein from the middle and lower part of the pig’s left or right ear, first disinfect it with tincture of iodine, then use 75% alcohol for deiodination and disinfection, and then use a No. 8 venous needle to collect blood 1 drop.

[0061] 1.2 Blood collection from the anterior vena cava: Find the depression in front of the pig’s right collarbone and right forelimb, first disinfect with tincture of iodine, then deiodine and disinfect with 75% alcohol, and then use a 20ml disposable syringe to add a blood collection needle to the anterior cavity Venous blood collection.

[0062] 2. Blood film specimen preparation

[0063] 2.1 Take 4 slides, 3 slides, and 1 slide (with smooth edges). Hold the two ends of the glass slide with the thumb and index finger of ...

experiment example

[0084] 1. The developed "Eperythrozoonis porcine rapid microscopic examination diagnostic kit" and "Eperythrozoon porcine PCR diagnostic kit" were compared for clinical diagnosis, through laboratory diagnosis and comparison of 850 blood samples. The accuracy rate of "Porcine Eperythrozoonosis Rapid Microscopic Examination Diagnostic Kit" is 98%, and the coincidence rate with "Porcine Eperythrozoonosis PCR Diagnostic Kit" is 98%.

[0085] 2. Using the "Rapid Microscopic Diagnosis Kit for Eperythrozoonosis in Porcine", from 2006 to 2011, nine cities and prefectures including Guiyang, Qiannan, Qiandongnan, Qianxinan, Liupanshui, Zunyi, Tongren, Anshun and Bijie were completed Epidemiological investigation of eperythrozoonosis in 1142 farms (households) in 133 townships, 443 villages (households) in 40 pig-raising base counties and 24 key pig-raising counties (cities, districts); the pathogen of eperythrozoon was found Wagyu Eperythrozoon pathogen. The morbidity rate of pigs rang...

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Abstract

A kit for rapid microscopic examination diagnosis of swine eperythrozoonosis and a preparing method thereof are provided. The kit includes detection reagents, vessels and instruments. The reagents include a Wright's stain, a Giemsa stain, glycerol, Tween-20 and methanol. The vessels and instruments include reagent bottles and droppers. The kit is scientific and reasonable in matching. A detectionmethod is simple, and detection accuracy is as high as 98% and is equivalent to accuracy of a swine eperythrozoonosis PCR diagnosis kit.

Description

Technical field: [0001] The invention belongs to the field of veterinary examination, and in particular relates to a rapid microscopic examination diagnostic kit for porcine eperythrozoonosis and a preparation method thereof. Background technique: [0002] Eperythrozoonosis is a zoonotic disease caused by Eperythrozoon parasitizing on the surface of red blood cells, plasma and bone marrow of humans or various animals. In the past, Eperythrozoa was classified as Rickettsia internationally, but in recent years, according to the 16SrRNA gene sequence analysis, Eperythrozoa is closer to Mycoplasma in classification. Eperythrozoonsuis (E. suis) is a pathogen parasitic on the surface of pig red blood cells or free in plasma, which can cause weakness and anemia in newborn and weaned piglets, slow growth in finishing pigs, reproductive disorders and low conception rate in sows Syndromic symptoms such as estrus, miscarriage and stillbirth seriously threaten the development of animal...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/28G01N1/31
Inventor 周思旋徐健徐洪忠肖芳萍黄波潘红
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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