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The method of inducing the formation of flower buds in A. microphylla test tube

An in-test tube, dragon bamboo technology, applied in the biological field, can solve problems such as short time consumption, achieve the effects of short time consumption, reduced economic losses, and simple operation

Active Publication Date: 2020-04-03
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

[0004] So far, there have been no reports in the prior art of successfully establishing a method for inducing the formation of flower buds in a test tube of A.

Method used

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  • The method of inducing the formation of flower buds in A. microphylla test tube
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  • The method of inducing the formation of flower buds in A. microphylla test tube

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Embodiment 1

[0014] 1. Establishment of sterile clones:

[0015] In 2009, the seeds of Dendrocalamus barbatus were collected in Mengla County, Xishuangbanna Dai Autonomous Prefecture, and a single-seed cluster-bud sterile clone was established through aseptic germination of the seeds, which was preserved in the germplasm resources of wild organisms in Southwest China in the form of test-tube cluster buds Library plant in vitro library. Concrete operation: peel off the outer testa of the collected A. microphyllum seeds, disinfect the surface with 75% alcohol for 1min, then clean it twice with distilled water for 5 minutes each time, and then use 0.1% mercuric chloride (HgCl 2 ) solution was sterilized for 10 min, washed with sterile distilled water for 5 times, and then the aseptic seeds were inoculated on the medium (1) 1 / 2MS to germinate. The basic medium is MS (Murashige & Skoog, 1962) (1 / 2MS means that the macroelements of MS medium are halved, and the rest of the components remain unc...

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Abstract

The invention provides a method for inducing the formation of flower buds in a dendrocalamus barbatus test tube. The method comprises the steps of selecting dendrocalamus barbatus sterile clustered vegetative buds, inoculating the vegetative buds to a flower bud induction culture medium, carrying out subculture once every 21 days, and carrying out three times of continuous flower bud induction culture and one time of recovery culture, so as to form the flower buds in the about 85th day. The method provided by the invention has the advantages that the operation is simple, the consumed time is short, the induction efficiency is high, and the repeatability is good; and a research material is provided for the prediction of the flowering of woody bamboo plants and the regulation and control offlowering time, and scientific guidance is provided for the decrease of the economic loss in the management of a bamboo forest.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for inducing the formation of flower buds in a test tube of A. microphylla. Background technique: [0002] Dendrocalamus barbatus Hsueh et D.Z.Li belongs to the Gramineae Bambusoideae genus and is a large sympodial cluster bamboo, mainly distributed in the tropical and subtropical regions of southern Yunnan, Vietnam, Myanmar and northern Laos. As an excellent bamboo fiber raw material, it has become one of the most promising economical bamboo species. However, the bamboo stalks wither after A. microphylla blooms. Even if individual bamboo clusters bloom in a bamboo forest, the entire bamboo forest will bloom and die within a few years, which will cause huge losses to the operators. Because there are no obvious signs of bamboo blooming in a large area, and it takes 8-10 years for natural renewal after flowering and death, it has caused troubles for practitioners...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 何俊李德铢程治英杨俊波李春芳李村富浦秀丽
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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