Method for prevention and treatment of rice sheath blight disease

A technology for rice sheath blight and sterile water is applied in the field of biological control of rice diseases and insect pests, which can solve problems such as singleness and achieve the effects of enhancing the control effect, increasing the permeability, and exerting the effect of biocontrol.

Inactive Publication Date: 2018-01-26
安徽省中日农业环保科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the single biological control in the prior art, the present invention provides a method for preventing and controlling rice sheath blight, which has a synergistic effect by using a mixture to control rice sheath blight

Method used

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Examples

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Embodiment 1

[0025] A control method for rice sheath blight, comprising the following steps:

[0026] Step 1: Prepare NA solid medium, use peptone 10.0g, beef powder 3.0g, sodium chloride 5.0g, agar 15.0g, glucose 2.5g, add distilled water to dissolve, adjust the volume to 1L, adjust the pH to 7±0.1, divide Sterilize at 121°C for 20 minutes after loading;

[0027] Step 2: Prepare LB liquid culture medium, add tryptone 10g, yeast extract 5g, and NaCl 10g in 950ml deionized water, stir until the solute dissolves, adjust the pH to 7.0 with 5mol / L NaOH, and dilute to 1L with deionized water. Steam sterilization under 15psi high pressure for 21min;

[0028] Step 3: Activate the antagonistic bacteria on the NA solid medium at 37°C for 24 hours, make a bacterial suspension with sterile water, take 10 mL of the bacterial suspension and inoculate it into a 250 mL Erlenmeyer flask containing 100 mL of LB liquid culture medium, Shake culture at a rate of 220 r / min for 48 hours at a constant tempera...

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Abstract

The invention discloses a method for prevention and treatment of rice sheath blight disease, and relates to the technical field of biological prevention and treatment of rice pest and disease damage;the method comprises the following steps: preparation of an NA solid culture medium, preparation of an LB liquid culture liquid, preparation of a seed liquid, preparation of a fermentation liquid, preparation of a fermentation liquid extract, preparation of a mixture, preparation of an mugwort extract, and rice fixed planting. The fermentation extract is mixed with validamycin to have a synergistic effect on prevention and treatment of the rice sheath blight disease, the prevention and treatment effect of validamycin on the rice sheath blight disease can be significantly enhanced, a certain theoretical basis is provided for the prevention and treatment effect of validamycin on the rice sheath blight disease, and an effective way is provided for expanding an application of antagonistic bacterial biological pesticides. Through an inhibitory characteristic of the mugwort extract on plant pathogenic bacteria, active substances in the traditional Chinese medicine mugwort are found out to have good inhibitory activity on the plant pathogenic bacteria.

Description

technical field [0001] The invention relates to the technical field of biological control of rice diseases and insect pests, in particular to a method for preventing and treating rice sheath blight. Background technique [0002] Rice sheath blight is a common disease that affects the growth of rice in my country. Generally, rice sheath blight will cause rice yield reduction by 10%-20%. my country's agricultural production has caused huge economic losses. [0003] However, at present, the effective agent for controlling rice sheath blight is relatively single, mainly Jinggangmycin. Due to long-term use, the rice sheath blight bacteria have developed a certain degree of resistance to Jinggangmycin, and the control effect of a single Jinggangmycin on rice sheath blight has been significantly weakened, making the number of times of use every year gradually increased, and the amount of use is also significant. Increase. Therefore, in order to improve the control effect of rice ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N43/16A01P3/00A01P21/00
Inventor 章云张贝尼
Owner 安徽省中日农业环保科技有限公司
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