Detergent composition
一种组合物、洗涤剂的技术,应用在洗涤剂组合物、表面活性洗涤剂组合物、洗涤剂配合剂等方向,能够解决汗渍没有被适当地去除、丢弃衣服等问题
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example 1
[0339] Materials and Methods
[0340] Isolation of specific bacterial strains from clothing
[0341] A strain of Brevundimonas sp. isolated from clothing was used in this example. Brevundomonas species were isolated during a study in which bacterial diversity in laundry after washing at 15°C, 40°C and 60°C was investigated. The study was performed on laundry collected from Danish households. For each wash, 20 g of laundry (tea towels, towels, dishcloths, overalls, base t-shirts, t-shirt collars, socks) in the range 4:3:2:2:1:1:1 were used. Washing was performed in a Laundr-O-Meter (LOM) at 15°C, 40°C or 60°C. For washing at 15°C and 40°C, use Ariel Sensitive White & Color laundry detergent (Ariel Sensitive White & Color), and for washing at 60°C, use WFK IEC-A* standard detergent. Ariel Sensitive White and Color Laundry Detergent was prepared by weighing out 5.1 g and adding tap water up to 1000 ml followed by stirring for 5 minutes. WFK IEC-A* standard detergent (avail...
example 2
[0351] Preparation of small swatches with biofilm
[0352] Brevundimonas sp. (isolated as described in Example 1) was incubated on Trypticase Soy Agar (TSA) (pH 7.3) (CM0131; Oxoid Ltd., Basingstoke, UK) at 30°C. Pre-grow for 2-5 days. A full circle from one single colony was transferred to 10 mL of TSB and incubated with shaking (240 rpm) at 30°C for 1 day. After propagation, Brevundimonas species were pelleted by centrifugation (Sigma Laboratory Centrifuge 6K15) (3000 g, 7 min at 21° C.) and resuspended in 10 mL of TSB diluted twice with water. Optical density (OD) at 600 nm was measured using a spectrometer (POLARstar Omega (BMG Labtech, Ortenberg, Germany)). Inoculate fresh TSB diluted twice with water to an OD of 0.03 600nm , and 1.6 mL was added to each well of a 12-well polystyrene flat-bottom microplate (3512; Corning Incorporated, Corning, NY, USA) in which no A small round swatch (diameter 2cm) of mycopolyester WFK30A. After incubation at 15° C. for 24 h with ...
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