An electrochemical sensor for detecting lead and its preparation method and application

An electrochemical and sensor technology, applied in the field of electrochemical sensors for detecting lead and its preparation, can solve the problems of affecting DNA activity, easy to use environmentally harmful substances, and unstable fixation, so as to improve sensitivity, high efficiency and sensitivity Effects of Sex, Affinity

Inactive Publication Date: 2018-02-23
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods all have defects such as weak fixation, the use of various affinity substances in fixation to affect the activity of DNA, and easy use of substances harmful to the environment.

Method used

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  • An electrochemical sensor for detecting lead and its preparation method and application
  • An electrochemical sensor for detecting lead and its preparation method and application
  • An electrochemical sensor for detecting lead and its preparation method and application

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Experimental program
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Embodiment 1

[0046] see figure 1 , an electrochemical sensor for detecting lead ions, comprising a glassy carbon electrode used as a working electrode in a three-electrode system, a signal amplification device, a response probe and a target probe.

[0047] Wherein, the surface of the detection end of the glassy carbon electrode is modified with ordered mesoporous carbon, nano gold particles are deposited on the ordered mesoporous carbon, and thiol-modified capture probes are connected to the nano gold particles.

[0048] The signal amplifying device includes ordered mesoporous carbon loaded with nano gold particles, and methylene blue is adsorbed on the ordered mesoporous carbon deposited with nano gold particles.

[0049] The response probe is modified on the signal amplification device through sulfhydryl group, and the nucleotide sequence of the response probe is the nucleotide sequence of deoxyribonucleic acid and nucleotide adenosine chimera. The desalted ribonucleic acid in the respo...

Embodiment 2

[0060] A preparation method of the electrochemical sensor of embodiment 1, specifically comprises the following steps:

[0061] 1. Preparation of ordered mesoporous carbon:

[0062] 1.1, the synthesis of mesoporous silicon template SBA-15: 8.0g block copolymer Pluronic P123 is placed in hydrochloric acid solution (hydrochloric acid solution is made of 270g H 2 O and 320mL, 1.54M hydrochloric acid), stirred in a 35°C water bath (the water bath temperature can be 30-35°C) until P123 dissolved, and then added 17g of tetraethyl orthosilicate (TEOS) dropwise , stirred at 35°C for 20h to obtain a mixed solution; transferred the mixed solution to a reaction kettle, heated it in a water bath at a temperature of 140°C for 24h (the water bath temperature can be 140°C to 150°C), and then carried out suction filtration to obtain the filtrate. The filtrate was washed with distilled water until neutral, and dried to obtain a white powder. Put the white powder into a box-type resistance fu...

Embodiment 3

[0079] The application of the electrochemical sensor of embodiment 1 in detecting lead ion, concrete detection method specifically comprises the following steps:

[0080] (1) Prepare the response probe and the signal amplification device into a solution with a response probe concentration of 2 μM, overnight at 4°C, centrifuge to remove the unadsorbed response probe, and redisperse to obtain the signal amplification modified by the response probe device.

[0081] (2) According to the volume ratio of 1:1, take the target probe with a concentration of 2 μM and react with the signal amplification device modified by the response probe modified by the sulfhydryl group obtained in step (1) for 30 minutes (reaction time can be implemented if the reaction time is 10 to 50 minutes) ), the signal amplifying device (S2 / S1 / MB / GNPs / OMC) modified by DNA double strands is obtained.

[0082] (3) Add the lead-containing solution to be tested to the signal amplification device obtained in step ...

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Abstract

An electrochemical sensor for detecting lead and its preparation method and application. The electrochemical sensor includes a glassy carbon electrode, a signal amplification device, a response probe and a target probe. The detection end of the glassy carbon electrode is modified with an ordered medium Porous carbon, and deposited with nano-gold particles, thiol-modified capture probes adsorbed on the nano-gold particles; the signal amplification device includes ordered mesoporous carbon deposited with nano-gold particles, ordered mesoporous carbon deposited with nano-gold particles methylene blue is adsorbed on the surface, and the response probe is adsorbed on the nano gold particle of the signal amplification device through thiol; the nucleotide sequence of the response probe is the nucleotide sequence of deoxyribonucleic acid and nucleotide adenosine chimera. The preparation method includes modifying the glassy carbon electrode, preparing a signal amplifying device and preparing a response probe and a target probe solution. The electrochemical sensor of the present invention is applied to detect lead ions in water bodies, and has the advantages of simplified operation, fast response, high sensitivity, high detection accuracy, strong anti-interference and the like.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to an electrochemical sensor for detecting lead and its preparation method and application. Background technique [0002] At present, the methods for determining pollutants in the environment mainly include chromatography, ultraviolet spectroscopy, synchronous fluorescence spectroscopy, spectrophotometry, derivative photometry, and flow injection analysis. These methods have defects such as complex pretreatment, long time consumption, large sample matrix effect, and long analysis cycle. They have high requirements on the operation level of instruments and staff, and it is difficult to popularize and apply them in small and medium-sized enterprises. For example, when using spectrophotometry to detect pollutants, due to the requirements for substrate turbidity and the influence of light-interfering substances, its accuracy and scope of use are limited; while liquid and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48
Inventor 曾光明竺园章毅汤琳程敏袁玉洁张立华
Owner HUNAN UNIV
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