Tissue culture propagation method for alpinia japonica

A technology of tissue culture and alpinia, which is applied in the field of plant tissue culture, can solve the problems of short growth cycle, unfavorable update of alpinia species, susceptibility to virus, etc., and achieve the effects of robust plant growth, remarkable disinfection effect, and robust root growth

Inactive Publication Date: 2018-02-09
广州普邦园林股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term propagation by ramets will easily lead to the accumulation of poor mutation traits, and is also susceptible to virus infection, which is not conducive to the renewal of "Red Harvest" Alpinia officinalis
The survival rate of tissue culture propagat...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present embodiment provides a tissue culture propagation method of Red Harvest Ginger, which comprises the following steps:

[0034]S1) Extraction and disinfection of explants: soak and disinfect the washed rhizome of Red Harvest Ginger with 800-fold dilution of carbendazim for 20 minutes, and place the sterilized rhizome of Red Harvest Ginger in a sterile, dark place at a temperature of In the moist sand at 25 ℃ for 15 days, during the germination of the underground stems of the Red Harvest Ginger, 800-fold dilutions of carbendazim should be sprayed once every 3 days; The leaf sheath wrapped by the outer layer of the new shoots, the pink shoots of 1 to 2 cm are used as explants, and the explants are sterilized;

[0035] S2) Adventitious bud induction culture: Inoculate the sterilized explants in step S1) into the adventitious bud induction medium to cultivate and induce adventitious buds; the adventitious bud induction medium contains 1mg / l 6-BA , 0.1mg / l NAA, 25g...

Embodiment 2

[0040] The present embodiment provides a tissue culture propagation method of Red Harvest Ginger, which comprises the following steps:

[0041] S1) Extraction and disinfection of explants: soak and disinfect the washed rhizome of Red Harvest Ginger with a 1000-fold dilution of carbendazim for 25 minutes, and place the disinfected rhizome of Red Harvest Ginger in a sterile, dark place at a temperature of 27 Cultivate germination in moist sand at ℃ for 13 days. During the germination cultivation period of the rhizomes of Alpinia officinalis, spray a 1000-fold dilution of carbendazim every 3 days; new shoots will germinate on the rhizomes of Alpinia officinalis after germination, and cut off the new shoots The leaf sheath wrapped by the outer layer uses 1-2cm pink shoots as explants, and the explants are sterilized;

[0042] S2) Adventitious bud induction culture: Inoculate the sterilized explants in step S1) into the adventitious bud induction medium to cultivate and induce adve...

Embodiment 3

[0047] The present embodiment provides a tissue culture propagation method of Red Harvest Ginger, which comprises the following steps:

[0048] S1) Extraction and disinfection of explants: soak and disinfect the washed rhizomes of Red Harvest Ginger with a 1100-fold dilution of carbendazim for 27 minutes, and place the sterilized rhizomes of Red Harvest Ginger in a sterile, dark place at a temperature of 26 Cultivate germination in moist sand at ℃ for 14 days. During the germination cultivation period of the underground stems of Alpinia officinalis, spray 1100 times of carbendazim dilution once every 3 days; new shoots will germinate on the underground stems of Alpinia officinalis after germination, and cut off the new shoots The leaf sheath wrapped by the outer layer uses 1-2cm pink shoots as explants, and the explants are sterilized;

[0049] S2) Adventitious bud induction culture: Inoculate the sterilized explants in step S1) into the adventitious bud induction medium to cu...

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PUM

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Abstract

The invention discloses a tissue culture propagation method for alpinia japonica and belongs to the technical field of plant tissue culture. The issue culture propagation method comprises the following steps: S1) taking and disinfecting an explant; S2) performing adventitious bud induction; S3) performing adventitious buds proliferation; S4) performing rooting culture; S5) hardening seedlings andtransplanting, wherein in the S1), subterraneous stems of the alpinia japonica are taken as raw materials and are disinfected for three times so as to obtain explant-pink buds. The alpinia japonica cultured by the method disclosed by the invention is short in cycle, fast in propagation and high in survival rate; meanwhile, the tissue culture propagation method disclosed by the invention can also be applied to Hongfengshou alpinia japonica.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and propagation of alpinia albicans. Background technique [0002] "Red Harvest" Harvest (Alpinia zerumbet×henryi'Harvest') is made of Alpiniazerumbet as the female parent and Alpiniahenryi as the male parent. The new evergreen perennial flower species of the genus Alpinia (Zingiberaceae) bred from the Zingiberaceae family has similar growth habits to the female parent Alpinia, and has strong adaptability to the natural environment in Guangdong. [0003] The plant height of "Hongfengshou" Ginger is 1.1-1.7m, and the leaves are 11-15 pieces. The leaves are dark green or light green, and have irregular radial dark spots; Hanging, 15-30cm long, 4-8cm in diameter, each inflorescence usually has 25-30 flower buds, and the inflorescence is relatively loose; the bracteoles are pink, and each bract has 2-3 small flowers; the flore...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 曾凤刘念黄邦海谭广文李秋静谢腾芳熊友华胡秀张施君叶向斌张学超
Owner 广州普邦园林股份有限公司
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