Preparation of a three-dimensional cell scaffold for in vitro toxicological evaluation of tobacco products and method for cell culture using it

Abstract

A method for preparing a three-dimensional cell scaffold for in-vitro toxicological evaluation of tobacco products is characterized in that an interconnected and porous material provided with a polymer is obtained through high-internal-phase emulsion polymerization in a cell culture dish, the material has large holes of the size of cells for in-vitro toxicological evaluation of tobacco products, the holes are communicated with one another, thereby facilitating input of oxygen and nutrients required for cell growth and output of cell metabolism waste, and the material surface is modified, so that the material has good cell affinity. Compared with existing panel type monolayer cell culture methods commonly adopted for toxicological evaluation, the method has the advantages that cells can realize three-dimensional growth on the scaffold and have more closer three-dimensional shape and biochemical and functional properties as compared with that originally in vivo. Therefore, when the cellscultured by use of the three-dimensional cell scaffold are used for in-vitro toxicological evaluation of the tobacco products, the evaluation result is more objective and real. The cell scaffold is prepared in the culture dish and is more convenient for following experiments.

Description

technical field

[0001] The invention belongs to the technical field of three-dimensional cell scaffold preparation, in particular to a method for preparing a three-dimensional cell scaffold for in vitro toxicological evaluation of tobacco products and using it for cell culture. Background technique

[0002] At present, the monolayer cell culture method is generally used in the in vitro toxicity evaluation of tobacco products. It has the advantages of simple culture, easy operation, low cost, and large-scale application. It is widely used as an experimental model for in vitro toxicology research of tobacco products. However, in monolayer cell culture, cells grow on the plane of the attached substrate. Due to the lack of three-dimensional scaffolds, they can only develop to two dimensions and cannot generate extracellular matrix. Unable to differentiate, thus losing the three-dimensional shape of the original body. Therefore, the biological characteristics reflected by monola...

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