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Endogenous penicillium fungus of radix liriopis proliferae and application thereof in preparation of steroidal saponin

A technology of Penicillium and Penicillium, which is applied in the field of microorganisms, can solve the problems of long production cycle of tuberous roots, environmental pollution of chemical synthesis, difficult synthesis, etc., and achieves the effects of avoiding unfavorable factors, avoiding pollution and large application value.

Active Publication Date: 2018-02-27
HUBEI UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although with the progress of chiral synthesis and oligosaccharide synthesis methods, there have been some reports on the total synthesis of Ophiopogon japonicus steroidal saponins, but due to the complexity of the steroidal saponin structure, the synthesis is very difficult, and the chemical synthesis is harmful to the environment. Huge pollution, currently mainly obtained directly through the separation of root extracts
However, in the actual production of Ophiopogon japonicus in Hubei, there are problems such as "small planting area of ​​continuous cropping obstacles", "greatly affected by climatic conditions", "long production cycle of roots", "low content of steroidal saponins", "high cost of saponin extraction". "And other factors, the market launch of Hubei Ophiopogon japonicus steroidal saponins is restricted
At present, there is no report on an endophytic fungus capable of producing Ophiopogon japonicus steroidal saponins through microbial fermentation and its application

Method used

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  • Endogenous penicillium fungus of radix liriopis proliferae and application thereof in preparation of steroidal saponin

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Experimental program
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Effect test

Embodiment 1

[0048] The Hubei Ophiopogon japonicus living tuber used for the isolation of the endophytic fungus EF100 strain of the present invention comes from the Hubei Ophiopogon japonicus GAP base, the origin of Hubei Ophiopogon japonicus in Oumiao Town, Xiangyang City, Hubei Province.

[0049] The endophytic fungus of the present invention is separated and obtained according to the following steps:

[0050] (1) Use a large amount of tap water to fully clean the soil on the surface of the root tuber, then put it into a clean beaker, add deionized water, and place it in an ultrasonic cleaner for repeated cleaning until the water after cleaning becomes extremely clear.

[0051] (2) After dipping the moisture on the surface of the tuber with sterile filter paper, proceed as follows: first soak the tuber with 75% (V / V) ethanol for 2 minutes, rinse it with sterile water for 3 times; Soak in -6% sodium hypochlorite solution for 2 minutes, then rinse continuously with a large amount of steril...

Embodiment 2

[0055] (1) Take the endophytic fungus EF100 bacterial classification of the present invention, under aseptic conditions, pick a small amount of hyphae with an inoculation needle, insert into a sterilized PDA medium test tube, and activate and cultivate at 28° C. for 72 hours.

[0056] (2) Take the activated cultured strain, transfer it into the sterilized liquid potato seed medium under aseptic conditions, and vibrate at 180 rpm at 28° C. for 72 hours to obtain seeds.

[0057] (3) prepare described liquid fermentation medium, pack in the Erlenmeyer flask of 250ml respectively, about 100ml of every bottle, sterilize, cool for standby; Under aseptic condition, insert seed according to 10% inoculum, 28 Cultivate with shaking at 180 rpm for 7 days.

[0058] (4) After the fermentation is completed, the fermented solid-liquid mixture is quickly frozen into a solid state in an ultra-low temperature refrigerator, and then subjected to low-temperature freeze-drying treatment using a sm...

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Abstract

The invention discloses an endogenous penicillium fungus of radix liriopis proliferae and application thereof in preparation of steroidal saponin, and relates to the technical field of microorganisms.The endogenous penicillium fungus of the radix liriopis proliferae and the application thereof in preparation of the steroidal saponin mainly solve a problem that the steroid saponin of the radix liriopis proliferae is limited in market due to factors of long tuberous production cycle, low steroidal saponin content and high extraction cost of saponin of the radix liriopis proliferae. The endogenous penicillium fungus is isolated from living roots of the radix liriopis proliferae (Liriope spicata var. prolifera). The endogenous penicillium fungus of the radix liriopis proliferae is identifiedby 18S rDNA sequence as Penicillium sp and is deposited with China Center for Type Culture Collection (CCTCC) on December 7, 2016 with the preservation number of CCTCC No. M2016724. According to the invention, the radix liriopis proliferae produced in radix liriopis proliferae source area, Oumiao town, Xiangyang city, Hubei Province-- radix liriopis proliferate GAP base is used, radix liriopis proliferae steroidal saponin is produced through liquid fermentation of endogenous penicillium fungus strains of the radix liriopis proliferae. The endogenous penicillium fungus is an important microorganism for searching for new resources of the radix liriopis proliferae steroidal saponin.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an endophytic fungus of Ophiopogon japonicus in Hubei, and the endophytic fungus is an endophytic Penicillium fungus capable of producing steroidal saponins from Ophiopogon japonicus in Hubei. Background technique [0002] Hubei Ophiopogon japonicus steroidal saponins, is a national geographical indication product, a genuine Chinese medicinal plant in Hubei - Ophiopogon japonicus from Hubei Liriope spicatavar. prolifera The main active ingredient of Y. T. Ma tuber root, its saponin basic skeleton belongs to the derivative of Spirostane (Spirostane, which has 27 carbon atoms in total), according to the configuration of C25 and the ring closure state of the ring in the Spirostane structure, it can be It is divided into spirostanols (C25 is S configuration), isospirostanols (C25 is R configuration), furostanols (F ring is an open-chain derivative), deformed spirostanols Alc...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12P33/20C12R1/80
CPCC12N1/02C12N1/14C12P33/20C12N1/145C12R2001/80
Inventor 余海忠叶国栋潘婉莲黄立明
Owner HUBEI UNIV OF ARTS & SCI
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