Endogenous penicillium fungus of radix liriopis proliferae and application thereof in preparation of steroidal saponin

Abstract

The invention discloses an endogenous penicillium fungus of radix liriopis proliferae and application thereof in preparation of steroidal saponin, and relates to the technical field of microorganisms.The endogenous penicillium fungus of the radix liriopis proliferae and the application thereof in preparation of the steroidal saponin mainly solve a problem that the steroid saponin of the radix liriopis proliferae is limited in market due to factors of long tuberous production cycle, low steroidal saponin content and high extraction cost of saponin of the radix liriopis proliferae. The endogenous penicillium fungus is isolated from living roots of the radix liriopis proliferae (Liriope spicata var. prolifera). The endogenous penicillium fungus of the radix liriopis proliferae is identifiedby 18S rDNA sequence as Penicillium sp and is deposited with China Center for Type Culture Collection (CCTCC) on December 7, 2016 with the preservation number of CCTCC No. M2016724. According to the invention, the radix liriopis proliferae produced in radix liriopis proliferae source area, Oumiao town, Xiangyang city, Hubei Province-- radix liriopis proliferate GAP base is used, radix liriopis proliferae steroidal saponin is produced through liquid fermentation of endogenous penicillium fungus strains of the radix liriopis proliferae. The endogenous penicillium fungus is an important microorganism for searching for new resources of the radix liriopis proliferae steroidal saponin.

Description

technical field

[0001] The invention relates to the technical field of microorganisms, in particular to an endophytic fungus of Ophiopogon japonicus in Hubei, and the endophytic fungus is an endophytic Penicillium fungus capable of producing steroidal saponins from Ophiopogon japonicus in Hubei. Background technique

[0002] Hubei Ophiopogon japonicus steroidal saponins, is a national geographical indication product, a genuine Chinese medicinal plant in Hubei - Ophiopogon japonicus from Hubei Liriope spicatavar. prolifera The main active ingredient of Y. T. Ma tuber root, its saponin basic skeleton belongs to the derivative of Spirostane (Spirostane, which has 27 carbon atoms in total), according to the configuration of C25 and the ring closure state of the ring in the Spirostane structure, it can be It is divided into spirostanols (C25 is S configuration), isospirostanols (C25 is R configuration), furostanols (F ring is an open-chain derivative), deformed spirostanols Alc...

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