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Liriope spicate endogenous schizophyllum fungus and application thereof to preparation of steroid saponin

A technology of steroidal saponins and Ophiopogon japonicus steroids, applied in the field of microorganisms, can solve the problems of small planting area, difficult synthesis, and long production cycle of tubers, and achieve the effects of avoiding pollution, great application value, and avoiding unfavorable factors

Active Publication Date: 2018-02-27
HUBEI UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although with the progress of chiral synthesis and oligosaccharide synthesis methods, there have been some reports on the total synthesis of steroidal saponin compounds, but due to the complexity of the steroidal saponin structure, the synthesis is very difficult, and the chemical synthesis is harmful to the environment. Huge pollution, currently mainly obtained directly through the separation of root extracts
However, in the actual production of Ophiopogon japonicus, there are problems such as "small planting area of ​​continuous cropping obstacles", "greatly affected by climatic conditions", "long production cycle of roots", "low content of steroidal saponins", "high cost of saponin extraction". " and other factors have led to restrictions on the market release of xiangmaidong steroidal saponins
At present, there is no report about a kind of endophytic fungus capable of producing xiangmaidong steroidal saponins through microbial fermentation and its application

Method used

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  • Liriope spicate endogenous schizophyllum fungus and application thereof to preparation of steroid saponin
  • Liriope spicate endogenous schizophyllum fungus and application thereof to preparation of steroid saponin

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Embodiment 1

[0048] The Xiangmaidong live tuber used for the isolation of the endophytic Schizophyllum fungus EF007 strain of the present invention comes from Xiangmaidong GAP base, the place of origin of Xiangmaidong in Oumiao Town, Xiangyang City, Hubei Province.

[0049] The endophytic fungus of the present invention is separated and obtained according to the following steps:

[0050] (1) Use a large amount of tap water to fully clean the soil on the surface of Xiangmaidong root tubers, then put it into a clean beaker, add deionized water, and place it in an ultrasonic cleaner for repeated cleaning until the water after cleaning becomes extremely clear;

[0051] (2) Use sterile filter paper to dip the water on the surface of the dry root tuber of Xiangmaodong, and then process it according to the following steps: first soak the tuber with 75% (V / V) ethanol for 2 minutes, rinse it with sterile water for 3 times; then use effective Soak in sodium hypochlorite solution with a chlorine cont...

Embodiment 2

[0055] The steps of preparing xiangmaidong steroidal saponins with the endophytic fungi obtained by the above method are as follows:

[0056] (1) Take the above-mentioned Xiangmaidong endophytic fungus EF007 strain, under aseptic conditions, pick a small amount of hyphae with an inoculation needle, insert it into a sterilized PDA medium test tube, and activate and cultivate it at 28°C for 72 hours ;

[0057] (2) Take the strains after the activated culture, transfer them into the sterilized liquid potato seed culture medium under aseptic conditions, and shake and cultivate them at 180 rpm for 72 hours at 28° C. to obtain seeds;

[0058] (3) Put the prepared liquid fermentation medium into 250ml Erlenmeyer flasks, about 100ml per bottle, sterilize and cool for later use; 180rpm shaking culture for 7 days;

[0059] (4) After the fermentation is completed, the fermented solid-liquid mixture is placed in an ultra-low temperature refrigerator and quickly frozen into a solid state...

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Abstract

The invention provides a liriope spicate endogenous schizophyllum fungus and application thereof to preparation of steroid saponin, and relates to the technical field of microorganisms. The fungus mainly solves the problems that due to the factors that the liriope spicate steroid saponin is mostly and directly from liriope spicate extracts, the liriope spicate steroid saponin content is low, the saponin extraction cost is high, and the like, the market delivery quantity of the liriope spicate steroid saponin is limited. The schizophyllum fungus is separated from living body tuberous roots of liriope spicata var. prolifera, and belongs to schizophyllum sp. through 18S rDNA sequence identification; the schizophyllum fungus is preserved in CCTCC (China Center for Type Culture Collection); thepreservation date is December 20th, 2016; the preservation number is CCTCC No. M2016765. The liriope spicate produced by a liriope spicate GAP (Good Agricultural Practices) base (the liriope spicatesource area in Oumao town, Xiangyang city in Hubei province ) is used; the liriope spicate steroid saponin is generated through liriope spicate endogenous fungus plant liquid fermentation. The endogenous fungus is an important microorganism for finding a new resource of the liriope spicate steroid saponin.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an endophytic fungus of Ophiopogon japonicus. The endophytic fungus is an endophytic fungus of the genus Schizophyllum capable of producing steroidal saponins from Ophiopogon japonicus. Background technique [0002] Xiangmaidong steroidal saponins are national geographical indication products, authentic Chinese medicinal plants in Hubei——Xiangmaidong Liriope spicatavar. prolifera The main active ingredient of Y. T. Ma tuber root, its saponin basic skeleton belongs to the derivative of Spirostane (Spirostane, which has 27 carbon atoms in total), according to the configuration of C25 and the ring closure state of the ring in the Spirostane structure, it can be It is divided into spirostanols (C25 is S configuration), isospirostanols (C25 is R configuration), furostanols (F ring is an open-chain derivative), deformed spirostanols Alcohols (F ring is a five-membered tetrah...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12P33/20C12R1/645
CPCC12N1/02C12N1/14C12P33/20C12N1/145C12R2001/645
Inventor 余海忠叶国栋潘婉莲黄立明
Owner HUBEI UNIV OF ARTS & SCI
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