A deep eutectic solvent for extracting ginkgo leaf proanthocyanidins and its preparation method and extraction method
A deep eutectic solvent and proanthocyanidin technology, applied in the field of agricultural and forestry biological resources utilization, can solve the problems of high requirements for supercritical fluid extraction equipment, easy volatilization of organic solvents, and operator injury, etc., achieve excellent solubility and conductivity, and improve extraction The effect of low efficiency and cost
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Embodiment 1
[0042] (1) Take by weighing the two components choline chloride and malonic acid of the deep eutectic solvent according to the molar ratio of 1:2.5 and mix them uniformly, heat and stir at 110° C. until a transparent liquid is formed;
[0043] (2) After the transparent liquid is formed, it is stored at 45° C. overnight. It is observed that the liquid has no solid precipitation, and the obtained deep eutectic solvent can be used for the extraction of ginkgo leaf proanthocyanidins.
Embodiment 2
[0045] (1) Take two components of deep eutectic solvent, choline chloride and malonic acid, and mix them uniformly according to a molar ratio of 1:2, and heat and stir at 100° C. until a transparent liquid is formed;
[0046] (2) After the transparent liquid is formed, it is stored at 50° C. overnight, and the liquid is observed without solid precipitation, and the obtained deep eutectic solvent can be used for the extraction of ginkgo biloba proanthocyanidins.
Embodiment 3
[0048] (1) Take two components of deep eutectic solvent, choline chloride and malonic acid, and mix them uniformly according to a molar ratio of 1:1.5, and heat and stir at 80°C until a transparent liquid is formed;
[0049] (2) After the transparent liquid is formed, it is stored at 45° C. overnight. It is observed that the liquid has no solid precipitation, and the obtained deep eutectic solvent can be used for the extraction of ginkgo leaf proanthocyanidins.
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