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One-dimensional calcium phosphate nano/micro material and its preparation method and application

A calcium phosphate, nanotechnology, applied in the field of gene carriers, can solve the problems of high cytotoxicity, difficult transfection research, easy agglomeration, etc.

Active Publication Date: 2021-06-15
SHENZHEN INST OF ADVANCED TECH
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Problems solved by technology

However, there are generally problems such as uncontrollable particle size, easy agglomeration, and low transfection efficiency.
However, the transfection efficiency of the calcium phosphate-gene co-precipitation method is very low (about 10-20%), and the prepared calcium phosphate particles have a large particle size and are difficult to control, have poor stability, and are easy to aggregate, so it is difficult to be used for in vivo transfection research.
Leaf Huang et al proposed a phospholipid bilayer-encapsulated DNA / calcium phosphate nanoparticle system prepared by the reverse-phase microemulsion method, but the purification steps for calcium phosphate are cumbersome, and the cationic liposomes used in the preparation process have high cytotoxicity , and the phospholipid material is expensive, which is not conducive to further clinical application

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  • One-dimensional calcium phosphate nano/micro material and its preparation method and application

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preparation example Construction

[0077] The invention provides a preparation method of a one-dimensional calcium phosphate nano / micro material, comprising the following steps:

[0078] (1) 3mLNaH 2 PO 4 solution (0.05mol / L) and 3mL of CaCl 2 The solution (0.1mol / L) was added to a mixed solvent composed of 15mL deionized water and 15mL absolute ethanol, and mixed uniformly at room temperature; then the pH value of the mixed solution was adjusted to 7.0±0.5 to obtain a reaction solution A. (2) Pour the reaction solution A into a microwave hydrothermal reaction kettle, heat it with microwave at 110°C for 10 minutes, and take out the reaction kettle when the system naturally cools below 60°C; (3) Centrifuge the product in the reaction kettle at 12000g 10 min, and the separated solid was washed 3 times with absolute ethanol, and finally freeze-dried to obtain a calcium phosphate nano / micro material, and this sample was named sample 1.

[0079] It should be noted that, according to different needs, the pH of sol...

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Abstract

The invention provides a one-dimensional calcium phosphate nanomaterial and its preparation method and application. The one-dimensional calcium phosphate nano / micro material is prepared by microwave-assisted hydrothermal reaction of water-soluble calcium salt and water-soluble phosphate. The one-dimensional Calcium vitamin phosphate nano / micro material is a rod-shaped structure with a length of 50nm-500μm and an aspect ratio greater than 5:1. It can be mixed with nucleic acid and delivered to the cell. It has good biocompatibility and is suitable for gene transfection. High efficiency and long duration.

Description

technical field [0001] The invention relates to the technical field of gene carriers, in particular to a one-dimensional calcium phosphate nano / micro material and its preparation method and application. Background technique [0002] Gene therapy achieves the purpose of treating diseases by introducing exogenous genes into the nucleus of target cells to repair defective genes that cause diseases or inhibit harmful genes that cause diseases. Successful gene therapy depends on effective gene carriers, and common carriers are divided into viral vectors and non-viral vectors. Viral vectors have limited their development because of the great potential safety hazards. In recent years, the emergence of minicircle DNA (Minicircle DNA, mcDNA) has become a highlight in gene vectors. Minicircle DNA is a circular expression box, which is obtained by removing the bacterial backbone DNA of the standard plasmid through DNA recombination technology. The product contains only one gene expre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B25/32B82Y30/00C12N15/87A61K47/02A61K48/00
CPCA61K47/02A61K48/0008B82Y30/00C01B25/325C01P2004/10C01P2004/61C01P2004/62C01P2004/64C12N15/87
Inventor 赵静陈国创黄萍王志勇何成宜陈志英
Owner SHENZHEN INST OF ADVANCED TECH
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