Light sheet illumination imaging device

An imaging device and light sheet technology, which can be used in measurement devices, fluorescence/phosphorescence, and material analysis through optical means, and can solve the problem of uneven imaging brightness

Pending Publication Date: 2018-04-06
北京拓澳生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The double-sided lighting technology can improve the imaging brightness unevenness and shadow problems to a certain extent, but it cannot completely solve the problem

Method used

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Embodiment Construction

[0022] The present invention will be described more fully hereinafter with reference to the accompanying drawings, in which exemplary embodiments of the invention are shown. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention.

[0023] see figure 1 In one embodiment, the light sheet illumination imaging device 100 includes a first achromatic lens 101, a second achromatic lens 102, a semi-cylindrical mirror 103, a mirror 104, a third achromatic lens 105, a micro Lens group 106 , illumination objective 107 , sample stage 108 , imaging objective 109 and imaging lens 110 .

[0024] The first achromatic lens 101 , the second achromatic lens 102 , the semicylindrical mirror 103 , and the mirror 104 are located on the same optical axis, and the light beam passes through the above elements to form the first optical axis. The reflective mirror 104, the t...

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Abstract

The invention relates to a light sheet illumination imaging device, which comprises a first achromatic lens, a second achromatic lens, a semi-cylinder lens, a reflecting mirror, a third achromatic lens, a micro lens group, an illumination objective lens, a sample table, an imaging objective lens and an imaging lens from the object side to the image side, wherein the first achromatic lens, the second achromatic lens, the semi-cylinder lens and the reflecting mirror are positioned on the same optical axis; the reflecting mirror, the third achromatic lens, the micro lens group, the illumination objective lens and the sample table are positioned on the same optical axis; the sample table, the imaging objective lens and the imaging lens are positioned on the same optical axis. The light sheet illumination imaging device has the advantages that through the combination and matching effect of one micro lens group and other components, the illumination light sheet can still form limited sheet layer light illumination in the illumination direction; parallel light can be changed into non-parallel crossed light; after the light passes through the illumination objective lens, the formed shade regions are blocked and absorbed in each direction of structures with low transparency in the sample on the sample table, so that shade formed by blocking and absorption can be eliminated.

Description

technical field [0001] The invention belongs to light sheet illumination imaging technology, in particular to a light sheet illumination imaging device. Background technique [0002] Light sheet illumination technology, also known as selective plan illumination, is a technique used in life science research to study large tissue blocks, plants and model organisms (such as zebrafish, nematodes, etc.) ) for fluorescence imaging. Light sheet imaging is to irradiate a specific layer of the sample with excitation illumination light in the direction perpendicular to the imaging direction (rather than the excitation and imaging coaxial as in wide-field or confocal imaging), and image the fluorescence excited by this layer. Compared with traditional confocal imaging, light sheet imaging has many advantages when imaging large / thick tissues, embryos, model animals and plants: 1. Lower fluorescence bleaching; 2. Lower sample damage; 3. Can Perform high-speed and high-signal-to-noise r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/645G01N2021/6463
Inventor 周旋何俊时令潘晓晨赵殊钢
Owner 北京拓澳生物科技有限公司
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