Dichloromethane extraction part of pepper and fructus piperis longi composition as well as preparation method and application of dichloromethane extraction part

A technology of dichloromethane and Piper longum, which is applied in the field of dichloromethane extraction and preparation of the core medicine of Hui medicine, Piper longum composition, can solve the problem of unspecified chemical composition of the medicine, abnormal urgency of stroke treatment drugs, Many adverse drug reactions

Inactive Publication Date: 2018-04-13
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] To sum up, the main problems existing in the prior art are: (1) Although the existing drugs for the treatment of stroke have a certain curative effect, but there are many adverse drug reactions, it is extremely urgent to find a safe and effective drug for the treatment of stroke; (2) The prior art points out that the combination of "pepper + longicorn" is the most widely used in the treatment of stroke, but it does not clearly indicate the effective part of the therapeutic effect and its medicinal chemical composition

Method used

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  • Dichloromethane extraction part of pepper and fructus piperis longi composition as well as preparation method and application of dichloromethane extraction part
  • Dichloromethane extraction part of pepper and fructus piperis longi composition as well as preparation method and application of dichloromethane extraction part
  • Dichloromethane extraction part of pepper and fructus piperis longi composition as well as preparation method and application of dichloromethane extraction part

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of dichloromethane extraction part of the pepper longan composition, its preparation method comprises the following steps:

[0034] S1: CO 2 Supercritical extraction and degreasing: Weigh 20kg each of pepper and longan, grind them in a grinder, mix them evenly, and put in CO 2 Extract in a supercritical extraction tank until no oil comes out, extraction conditions: pressure 300Bar; temperature 55°C, separation tank I pressure 80Bar, separation I temperature 50°C, separation tank II pressure 60Bar, separation II temperature 40°C.

[0035] S2: Ethanol extraction: add the medicinal residues obtained in step S1 into the multifunctional extraction tank several times, and extract with 70% ethanol for 3 times, each time for 3 hours, and the volume ratio of medicinal residues:70% ethanol is 1:10. Combine the extracts, place them in a rotary evaporator, and spin until there is no alcohol smell to obtain a crude ethanol extract solution.

[0036] S3: dichloromethane extr...

Embodiment 2

[0038] On the basis of Example 1, the early protective effect experiment on rat cerebral ischemia recovery was carried out on the dichloromethane extraction part obtained from Example 1.

[0039] 1 Experimental materials

[0040] 1.1 Main reagents and consumables

[0041] A4-2026 thread tie; chloral hydrate; 95% alcohol; distilled water; sodium carboxymethylcellulose (CMC-Na); malondialdehyde MDA detection kit.

[0042] 1.2 Main Instruments

[0043] YLS-12A Rat and Mouse Grip Tester; Electronic Analytical Balance.

[0044] 2. Experimental Animals and Methods

[0045] Male healthy SD rats, weighing 280-320 g, were provided by the Experimental Animal Center of Ningxia Medical University.

[0046] The middle artery of rats was blocked by suture method, and the model of permanent cerebral ischemic injury in rats was established. Rats with successful surgical models were randomly divided into groups (10-15 rats in each group): model group, sham operation group, high dichlorome...

Embodiment 3

[0084] Preparation of ordinary tablets:

[0085] On the basis of Example 1, the dichloromethane extraction fraction obtained in Example 1 is mixed with auxiliary materials at a weight ratio of 1:5, granulated, and tabletted to obtain the product.

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Abstract

The invention belongs to the technical field of medicine preparation, and relates to a chemical component extraction and separation method for medicinal plants, in particular to a dichloromethane extraction part of a pepper and fructus piperis longi composition as well as a preparation method and application of the dichloromethane extraction part. The method comprises the following steps: weighingpepper and fructus piperis longi according to a weight ratio ranging from (1:10) to (10:1), smashing and uniformly mixing the pepper and the fructus piperis longi, performing supercritical extractionand oil removal, extracting herb residues with ethyl alcohol at the concentration of 70 percent, performing vacuum concentration on an extracting solution till no alcohol odor exists, performing extraction with dichloromethane, recycling the dichloromethane in the extracting solution to obtain a fluid extract, and drying the fluid extract to obtain the dichloromethane extraction part. The dichloromethane extraction part contains 10 compounds such as piperidine butyrylamide G. Pharmacodynamical experimental results show that the dichloromethane extraction part of the pepper and fructus piperislongi composition has a brain protection effect on early recovery of cerebral ischemia of a rat; the dichloromethane extraction part of the pepper and fructus piperis longi composition and the preparation method have extremely high academic and application value.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and relates to a method for extracting and separating medicinal phytochemical components, in particular to a dichloromethane extraction part of the core medicine of back medicine, a composition of Piper longum and longan, and a preparation method and application thereof. Background technique [0002] Stroke is an acute cerebrovascular disease characterized by disturbance of consciousness and focal neurological deficit. Stroke has a high incidence rate, high recurrence rate, and high disability rate, and has become the primary factor of national death. The 2016 "China Stroke Prevention Report" shows that there are more than 2 million new stroke patients in my country every year; more than 1.8 million patients die of stroke every year, and most of the surviving patients also cause disabilities, which brings great harm to the family and society. Big burden. The Chinese government direc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/67A61P9/10
CPCA61K36/67A61K2236/333A61K2236/37A61K2236/39A61K2236/55A61K2300/00
Inventor 付雪艳花施瑶郭东燕王兵董琳李婷婷黄俊陈容马嘉华
Owner NINGXIA MEDICAL UNIV
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