Dichloromethane extraction part of pepper and fructus piperis longi composition as well as preparation method and application of dichloromethane extraction part
A technology of dichloromethane and Piper longum, which is applied in the field of dichloromethane extraction and preparation of the core medicine of Hui medicine, Piper longum composition, can solve the problem of unspecified chemical composition of the medicine, abnormal urgency of stroke treatment drugs, Many adverse drug reactions
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Embodiment 1
[0033] A kind of dichloromethane extraction part of the pepper longan composition, its preparation method comprises the following steps:
[0034] S1: CO 2 Supercritical extraction and degreasing: Weigh 20kg each of pepper and longan, grind them in a grinder, mix them evenly, and put in CO 2 Extract in a supercritical extraction tank until no oil comes out, extraction conditions: pressure 300Bar; temperature 55°C, separation tank I pressure 80Bar, separation I temperature 50°C, separation tank II pressure 60Bar, separation II temperature 40°C.
[0035] S2: Ethanol extraction: add the medicinal residues obtained in step S1 into the multifunctional extraction tank several times, and extract with 70% ethanol for 3 times, each time for 3 hours, and the volume ratio of medicinal residues:70% ethanol is 1:10. Combine the extracts, place them in a rotary evaporator, and spin until there is no alcohol smell to obtain a crude ethanol extract solution.
[0036] S3: dichloromethane extr...
Embodiment 2
[0038] On the basis of Example 1, the early protective effect experiment on rat cerebral ischemia recovery was carried out on the dichloromethane extraction part obtained from Example 1.
[0039] 1 Experimental materials
[0040] 1.1 Main reagents and consumables
[0041] A4-2026 thread tie; chloral hydrate; 95% alcohol; distilled water; sodium carboxymethylcellulose (CMC-Na); malondialdehyde MDA detection kit.
[0042] 1.2 Main Instruments
[0043] YLS-12A Rat and Mouse Grip Tester; Electronic Analytical Balance.
[0044] 2. Experimental Animals and Methods
[0045] Male healthy SD rats, weighing 280-320 g, were provided by the Experimental Animal Center of Ningxia Medical University.
[0046] The middle artery of rats was blocked by suture method, and the model of permanent cerebral ischemic injury in rats was established. Rats with successful surgical models were randomly divided into groups (10-15 rats in each group): model group, sham operation group, high dichlorome...
Embodiment 3
[0084] Preparation of ordinary tablets:
[0085] On the basis of Example 1, the dichloromethane extraction fraction obtained in Example 1 is mixed with auxiliary materials at a weight ratio of 1:5, granulated, and tabletted to obtain the product.
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