Glioma prognostic marker hsa_circ_0125361 and its application

A technology for glioma and prognosis, applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve the problems that the survival rate of glioma patients has not been significantly improved, and the survival rate is not optimistic

Active Publication Date: 2020-06-30
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic

Method used

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  • Glioma prognostic marker hsa_circ_0125361 and its application
  • Glioma prognostic marker hsa_circ_0125361 and its application
  • Glioma prognostic marker hsa_circ_0125361 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Preparation of circRNA hsa_circ_0125361 kit for the prognosis of glioma patients (50 reactions)

[0017] 1. RNA stabilization solution 50ml

[0018] 2. Isopropanol 100ml

[0019] 3. Chloroform 100ml

[0020] 4. Trizol 50ml

[0021] 5. Enzyme-free water 10ml

[0022] 6. 1μM random reverse transcription primer 50μl

[0023] 7. 5× reverse transcription buffer 200ml

[0024] 8. 100μl of 10mM base triphosphate deoxyribonucleotides

[0025] 9. 40U / μl RNase inhibitor 500μl

[0026] 10. 200U / μl MMLV reverse transcriptase 50μl

[0027] 11.Premix Ex Taq 50μl

[0028] 12. 10μM circRNA hsa_circ_0125361 real-time fluorescence quantitative PCR specific primer 30μl

[0029] circRNA hsa_circ_0125361 forward primer: 5'-CCAAGCAGCTCACTACGATA-3',

[0030] circRNA hsa_circ_0125361 reverse primer: 5'-CAAGCAGGTAGGAGATTCCA-3';

[0031] 13. 10μM GAPDH specific primer 30μl

[0032] The forward primer is 5′-ATCATCAGCAATGCCTCCT-3′,

[0033] The reverse primer was 5'-CATCACG...

Embodiment 2

[0034] Example 2: The relationship between the expression level of circRNA hsa_circ_0125361 in glioma tissue and prognosis

[0035] 1. Collect the glioma tissue to be tested, put it into a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0036] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After the liquid was transferred to a tube, it was lysed on ice for 15 minutes. After the lysis, centrifuge at 12000rpm for 10min at 4°C, and transfer the supernatant to a new tube. Add 200 μl of chloroform to the tube, shake it by hand for 15-30 seconds, place on ice for 5 minutes, centrifuge at 12,000 rpm for 15 minutes at 4°C; carefully take the upper layer of the aqueous phase into a new tube, add 0.5ml of pre-cooled isopropanol a...

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Abstract

The invention discloses a glioma prognostic marker hsa_circ_0125361 and application. A reagent for detecting circRNA hsa_circ_0125361 of a glioma source is used for preparing a prognostic preparationof a glioma patient. Research proves that the glioma patient with relatively high expression of circRNA hsa_circ_0125361 has relatively high postoperative survival rate. A prognosis of the glioma patient is made by detecting the expression level of circRNA hsa_circ_0125361 in glioma tissue of the glioma patient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a circRNA marker for glioma prognosis, the application of a reagent for detecting the marker to prepare a glioma prognosis preparation, and a kit. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 武明花许梓豪李沛瑶余志斌张严刘强陈琼
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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