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Application, kit and detection method of novel molecular marker non-coding RNA LINC00922 for prognosis of kidney cancer

A molecular marker, non-coding technology, applied in the field of tumor molecular biology, to achieve far-reaching clinical significance, improve postoperative quality of life, and improve the effect of survival rate

Inactive Publication Date: 2018-10-12
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether LINC00922 can be used as a new tumor marker to predict the prognosis of renal cancer patients has not been reported

Method used

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  • Application, kit and detection method of novel molecular marker non-coding RNA LINC00922 for prognosis of kidney cancer
  • Application, kit and detection method of novel molecular marker non-coding RNA LINC00922 for prognosis of kidney cancer
  • Application, kit and detection method of novel molecular marker non-coding RNA LINC00922 for prognosis of kidney cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: The application of non-coding RNA LINC00922, a novel molecular marker for prognosis of renal cancer, in the preparation of preparations for predicting the prognosis of renal cancer patients.

Embodiment 2

[0021] Example 2: Preparation of reagents for detecting the expression of non-coding RNA LINC00922 for the preparation of a kit for the prognosis of renal cancer patients (for 30 reactions) 1. Trizo 120ml

[0022] 2. Inhibit RNA degradation solvent 40ml;

[0023] 3. Chloroform 80ml;

[0024] 4. 80ml of isopropanol;

[0025] 5. DEPC water 10ml;

[0026] 6. 100 μl of a mixture of 10× random primers and Oligo dT primers;

[0027] 7. 150 μl of 5× reverse transcription reaction buffer;

[0028] 8. 100μl of 10mM deoxyribonucleotide triphosphate base dNTP containing Mg2+;

[0029] 9. 200U / μl M-MLV reverse transcriptase 50μl;

[0030] 10. SYBR Green qPCR Mix 500μl;

[0031] 11. 3μM target gene LINC00922 specific primer (its sequence is shown in SEQ NO:2 and SEQ NO:3) 50μl;

[0032] 12. 50 μl of 3 μM internal reference gene TUBA1A-specific primer (its sequence is shown in SEQ NO:4 and SEQ NO:5).

Embodiment 3

[0033] Example 3: Detection of non-coding RNA LINC00922 in tissue samples

[0034] 1. Collect the kidney cancer or normal control tissues to be tested, wash them with normal saline, put them into a cryopreservation tube filled with a solvent that inhibits RNA degradation, and put them in a -80°C refrigerator for later use.

[0035] 2. Extraction of RNA in tissues:

[0036] (1) Add liquid nitrogen to the mortar first, then cut the tissue into small pieces and grind it into powder in liquid nitrogen, take 100mg of tissue powder with a liquid nitrogen pre-cooled spoon and add it to the EP tube filled with 1ml of Trizol solution middle. The total volume of tissue powder should not exceed 10% of the volume of Trizol used, and it should be mixed thoroughly;

[0037] (2) Leave at room temperature for 5 minutes, then add 200 μl of chloroform, tightly cap the EP tube and shake vigorously for 0.5 minutes. Centrifuge at 12000 rpm for 10 minutes;

[0038] (3) Take the upper aqueous ph...

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Abstract

The invention relates to application, a kit and a detection method of novel molecular marker non-coding RNA LINC00922 for prognosis of kidney cancer. Real-time fluorescence quantitative PCR (Polymerase Chain Reaction) and survival curve analysis show that the non-coding RNA LINC00922 derived from kidney cancer tissues is related with a survival rate of a patient, the content is higher and the survival rate is higher. The method provides powerful technical support for predicting prognosis survival rate of kidney cancer patients, the postoperative life quality of the kidney cancer patients is facilitated, the developing of a postoperative treatment scheme is facilitated, the survival rate is increased, and the application, the kit and the detection method of the novel molecular marker non-coding RNA LINC00922 for prognosis of the kidney cancer disclosed by the invention have profound clinical significance.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and in particular relates to an application, a kit and a detection method of a novel molecular marker non-coding RNA LINC00922 for prognosis of kidney cancer. Background technique [0002] Renal cell carcinoma is a malignant tumor originating from the urothelial system of the renal parenchyma. It is a common tumor in the genitourinary system, and its prevalence accounts for 3% of all malignant tumors in the whole body. In recent years, its incidence has been increasing year by year. In my country, the incidence rate is about 5 / 100,000, the mortality rate is 1.5 / 100,000, and the ratio of male to female incidence is about 2:1. It has many pathological types, and the most important pathological type is renal clear cell carcinoma, which accounts for about 70% of the total renal cancer, which is better than middle-aged and elderly people. The etiology of kidney cancer is unknown, but the curre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6851C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178C12Q2531/113C12Q2563/107C12Q2545/101C12Q2561/113
Inventor 田田
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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