Combination method of NAP2 polyclonal antibody and liver cancer serum NAP2

Abstract

The invention discloses a combination method of an NAP2 polyclonal antibody and liver cancer serum NAP2; combination of the NAP2 polyclonal antibody and the liver cancer serum NAP2 comprises the steps: the NAP2 polyclonal antibody is added to the serum of liver cancer patients, and SELDI-TOF-MS detection of an antigen and antibody combination condition is carried out; after antigen and antibody specific combination, peaks of a liver cancer serum specific marker protein 7789Da and 7984Da protein are decreased obviously; and the prepared NAP2 polyclonal antibody is indicated to be specifically combined with the liver cancer serum marker protein 7789Da and 7984Da protein, and the liver cancer serum marker protein 7789Da and 7984Da protein are also indicated to be different enzymolysis fragments of NAP2. Searching of effective tumor markers for researches of canceration mechanism and early diagnosis and prognosis judgment of diseases is of great significance.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to a method for combining NAP2 polyclonal antibody with liver cancer serum NAP2. Background technique [0002] In the 20th century, primary liver cancer has risen to become the second cancer killer in my country, second only to lung cancer in urban areas and gastric cancer in rural areas [1] . At present, about 35,000 people in Guangxi die of malignant tumors every year, among which, about 12,000 people die of liver cancer. It can be seen that liver cancer is the first cancer killer in Guangxi. In Guangxi, the hazards of liver cancer are very uneven. The counties and cities with high mortality rate of liver cancer are mainly concentrated in the southwestern part of Guangxi. The three counties of Fusui, Long'an and Wuming around Nanning have the highest incidence. The incidence density of liver cancer in this local area reaches 50-60 / 100,000. In some administrat...

AI Technical Summary

Problems solved by technology

The high sensitivity of mass spectrometry can help people discover low-abundance proteins and peptides in biological samples in the stage of marker discovery. The dynamic range greatly affects the quantitative detection of low-abundance proteins and peptides in biological fluids by mass spectrometry

Although researchers have tried to use a variety of methods, including chromatographic separation as much as possible, removal of high-abundance proteins, etc., the precision and accuracy of the results are still far from meeting the requirements of analysis, until mass spectrometry multiple reaction monitoring (multiple reaction monitoring) The emergence of monitoring, MRM) technology

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