Method for detecting expression of NAP2 in liver cancer and para-carcinoma tissue through immunohistochemical detection

A technique of paracancerous tissue and immunohistochemistry, applied in the fields of gene transcriptomics and proteomics, can solve the problems affecting the quantitative detection of low-abundance proteins, and the precision and accuracy cannot meet the analysis requirements

Inactive Publication Date: 2018-04-13
南宁科城汇信息科技有限公司
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Problems solved by technology

The high sensitivity of mass spectrometry can help people discover low-abundance proteins and peptides in biological samples in the stage of marker discovery. The dynamic range greatly affects the quantitative detection of low-abundance proteins and peptides in biological fluids by mass spectrometry
Although researchers have tried to use a variety of methods, including chromatographic separation as much as possible, removal of high-abundance proteins, etc., the precision and accuracy of the results are still far from meeting the requirements of analysis, until mass spectrometry multiple reaction monitoring (multiple reaction monitoring) The emergence of monitoring, MRM) technology

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  • Method for detecting expression of NAP2 in liver cancer and para-carcinoma tissue through immunohistochemical detection

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Embodiment

[0019] Consists of the following steps:

[0020] Immunohistochemical detection of NAP2 expression in liver cancer and paracancerous tissues: the results are as follows figure 1 As shown, NAP2 is highly expressed in liver cancer tissues, and basically has no expression or very low expression in paracancerous control tissues.

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Abstract

The invention discloses a method for expression of NAP2 in liver cancer and para-carcinoma tissue through immunohistochemical detection. The immunohistochemical detection result of expression of NAP2in liver cancer and para-carcinoma tissue is as shown in figure 1 in the specification, NAP2 is highly expressed in liver cancer tissue, and is barely expressed or very lowly expressed in para-carcinoma reference tissue. The invention searches an effective tumor marker which has great significances for tumorigenesis mechanism study and early-stage diagnosis and prognosis on diseases. Bioinformatics analysis shows that functions of key proteins in system signal networks are analyzed, furthermore through MRM (Multiple Reaction Monitoring) absolute quantification verification, liver cancer diagnosis values of liver cancer key protein polypeptide are evaluated, clinic correlation is analyzed, and feasibility of the key proteins in clinical application such as liver cancer prevention, early-stage diagnosis and prognosis is put forward.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to a preliminary method for detecting the expression of NAP2 in liver cancer and adjacent tissues by immunohistochemistry. Background technique [0002] Since primary liver cancer generally has no symptoms in the early stage, once clinical manifestations appear, most of the disease has entered the middle and late stages, and the degree of malignancy is high, the progression is fast, the prognosis is poor, and the invasion is strong. Therefore, it is necessary to find effective tumor markers for canceration. Mechanism research, drug target design, early diagnosis of disease and screening of high-risk groups have become the top priority of liver cancer research in Guangxi. [0003] At present, the detection and diagnosis of liver cancer is based on serum AFP detection combined with imaging examination. Among them, the more clinically recognized diagnostic criteria ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57438G01N33/57488
Inventor 于思创王海云
Owner 南宁科城汇信息科技有限公司
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