Antibody preparation and titer detection method

Abstract

The invention discloses an antibody preparation and titer method. According to the comprehensive analysis of the DNAstrart software, the NAP2 has high hydrophilicity, basically contains two flexible regions, and has a high score of antigen fingerprints, and is selected as the antigen polypeptide; through the synthesis of the polypeptide, the antigen Modification, animal immunization, antibody purification, concentration measurement, lyophilized storage, sodium dodecylsulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis Coomassie blue test body antibody purity; looking for effective tumor markers It is of great significance to use drugs in the study of carcinogenesis mechanism, early diagnosis and prognosis of diseases. Evaluate the diagnostic value of these key liver cancer protein polypeptides and analyze their clinical relevance, and propose their clinical application feasibility in the prevention, early diagnosis and prognosis of liver cancer.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to an antibody preparation and titer method. Background technique [0002] In the 20th century, primary liver cancer has risen to become the second cancer killer in my country, second only to lung cancer in urban areas and gastric cancer in rural areas [1] . Since primary liver cancer generally has no symptoms in the early stage, once clinical manifestations appear, most of the disease has entered the middle and late stages, and the degree of malignancy is high, the progression is fast, the prognosis is poor, and the invasion is strong. Therefore, it is necessary to find effective tumor markers for canceration. Mechanism research, drug target design, early diagnosis of disease and screening of high-risk groups have become the top priority of liver cancer research in Guangxi. [0003] At present, the detection and diagnosis of liver cancer is based on serum AFP d...

AI Technical Summary

Problems solved by technology

The high sensitivity of mass spectrometry can help people discover low-abundance proteins and peptides in biological samples in the stage of marker discovery. The dynamic range greatly affects the quantitative detection of low-abundance proteins and peptides in biological fluids by mass spectrometry

Although researchers have tried to use a variety of methods, including chromatographic separation as much as possible, removal of high-abundance proteins, etc., the precision and accuracy of the results are still far from meeting the requirements of analysis, until mass spectrometry multiple reaction monitoring (multiple reaction monitoring) The emergence of monitoring, MRM) technology

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