Cross method of indirect ELISA method

Abstract

The invention discloses a cross method of an indirect ELISA method; crossing is performed according to the indirect ELISA method; OD curves of a cross control and a negative control are basically similar and are close to zero level; an anti-liver cancer differential protein Nap2 antibody is indicated to not exist (or be less expressed) in pre-immunized serum; rabbit anti HO-1 and anti SPA60 polyclonal immune sera cannot react with a liver cancer differential protein NAP2 antigen, and the specificity of the antigen is further proved; an OD curve of the anti-NAP2 immune serum is obviously higherthan the OD curves of the cross control and the negative control, moreover, the OD value presents a significant dropping trend with the decrease of the serum concentration. Therefore, a large numberof corresponding antibodies are believed to be produced in domestic rabbit serum immunized with NAP2, and the specificity of the antibodies is further confirmed. Searching of effective tumor markers for researches of canceration mechanism and early diagnosis and prognosis judgment of diseases is of great significance.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to a crossover method of indirect ELISA. Background technique [0002] Since primary liver cancer generally has no symptoms in the early stage, once clinical manifestations appear, most of the disease has entered the middle and late stages, and the degree of malignancy is high, the progression is fast, the prognosis is poor, and the invasion is strong. Therefore, it is necessary to find effective tumor markers for canceration. Mechanism research, drug target design, early diagnosis of disease and screening of high-risk groups have become the top priority of liver cancer research in Guangxi. [0003] At present, the detection and diagnosis of liver cancer is based on serum AFP detection combined with imaging examination. Among them, the more clinically recognized diagnostic criteria for liver cancer are: serum AFP concentration > 400 μg / L for 4 weeks, imaging f...

AI Technical Summary

Problems solved by technology

The high sensitivity of mass spectrometry can help people discover low-abundance proteins and peptides in biological samples in the stage of marker discovery. The dynamic range greatly affects the quantitative detection of low-abundance proteins and peptides in biological fluids by mass spectrometry

Although researchers have tried to use a variety of methods, including chromatographic separation as much as possible, removal of high-abundance proteins, etc., the precision and accuracy of the results are still far from meeting the requirements of analysis, until mass spectrometry multiple reaction monitoring (multiple reaction monitoring) The emergence of monitoring, MRM) technology

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