Kit for detecting heredity and susceptibility mitochondria deafness genes

Abstract

The invention provides a kit for detecting heredity and susceptibility mitochondria deafness genes related to a mitochondrial gene. The kit comprises a nucleic acid membrane strip and multiple PCR primers, wherein the nucleic acid membrane strip comprises a substrate made of a nylon membrane material and a specific oligonucleotide probe fixed on the substrate; the sequences of the probe are shownin SEQ ID NO:1-43; and the sequences of the multiple PCR primers are shown in SEQ ID NO:44-59. The detection kit provided by the invention can detect mutation of 22 deafness genes on the same nucleicacid membrane strip simultaneously by a reverse dot blot (RDB) technology. The kit covers the common mutation sites of the mitochondria deafness genes, can complete detection in the same day, and is amitochondria deafness virulence gene detection kit with high cost performance, high practicability, rapidness and convenience.

Description

technical field [0001] The invention belongs to the field of gene detection of life science and technology, and specifically relates to a kit for detection of hereditary and susceptibility mitochondrial deafness genes by the classical RDB method. Background technique [0002] Deafness is the most common sensory defect disease in humans. There is about 1 case of deafness in every 500 newborns, and there are 278 million deafness patients worldwide. According to my country's second national sample survey of disabled people in 2006, there were 27.8 million people with hearing disabilities, accounting for 33.5% of the country's 82.96 million disabled people, accounting for 25% of the world's hearing-impaired population, ranking first in disability diseases. Among them, 800,000 hearing-impaired children aged 0-6, 110,000 hearing-impaired children aged 6-14, and >30,000 newborn deaf children each year (excluding delayed deafness and drug-induced deafness). Mitochondrial deafnes...

AI Technical Summary

Problems solved by technology

Each simultaneous detection of an additional site increases the difficulty of probe and primer design

In view of the fact that there are few detection sites for mitochondrial DNA deafness products on the market, and the diversity and high incidence of mitochondrial deafness mutation sites, it is urgent to establish a high-throughput, high-efficiency, economical and rapid detection method and product for mitochondrial deafness genes. Realize rapid clinical detection or large-scale population screening

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