Streptococcus pneumoniae with lytr function deficiency, vaccine, preparation method and application thereof

Abstract

This patent discloses a Streptococcus pneumoniae with LytR function deficiency, the Streptococcus pneumoniae is a Streptococcus pneumoniae serotype strain, and the genome of the strain contains SPD_1741 with overall deletion, partial deletion, sequence point mutation or sequence point deletion Gene, the SPD_1741 gene includes itself and its homologous genes or alleles. Also disclosed is a method for preparing the LytR functionally deficient Streptococcus pneumoniae, using the LytR protein as an antibiotic target for Streptococcus pneumoniae or other bacteria, and using the LytR functionally deficient Streptococcus pneumoniae as a target for preventing, alleviating or treating Streptococcus pneumoniae infection. For the application of vaccines, the present invention uses the SPD_1741 gene itself and its homologous genes or alleles to interfere with or inhibit the expression of the SPD_01741 gene and its encoded products due to factors such as overall deletion, partial deletion, sequence point mutation or sequence point deletion, Thereby interfering or destroying the function of SPD_01741 gene or the loss of activity makes LytR function defective.

Description

technical field [0001] The present invention relates to the field of medical biotechnology, in particular to a Streptococcus pneumoniae with LytR functional deficiency, a vaccine and its preparation method and application, and also relates to an antibiotic target acting on Streptococcus pneumoniae and Streptococcus pneumoniae with LytR functional deficiency as a preventive Vaccines for pneumococcal infection. Background technique [0002] Streptococcus pneumoniae (S.pn) usually resides on the mucosal surface of the human nasopharynx. It is an opportunistic pathogen that can cause pneumonia, sepsis, meningitis or otitis media. The total number of deaths due to Streptococcus pneumoniae infection is as high as 3 to 5 million people in the world every year, especially children and the elderly. Among them, the annual incidence of children under 5 years old is as high as 1 million, and the mortality rate is as high as 11%. [0003] In the treatment of Streptococcus pneumoniae inf...

AI Technical Summary

Problems solved by technology

[0006] The present invention intends to provide a Streptococcus pneumoniae with LytR functional deficiency and its preparation method, and an antibiotic target for Streptococcus pneumoniae, so as to solve the problems of drug resistance and expensive vaccines that are difficult to popularize in the treatment of Streptococcus pneumoniae infection

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