Streptococcus pneumoniae deficient in LytR function, vaccine and preparation method and application thereof

Abstract

The invention discloses a streptococcus pneumoniae deficient in the LytR function. The streptococcus pneumoniae is a serotype strain of streptococcus pneumoniae. The genome of the strain includes a SPD_1741 gene with entire deletion, partial deletion, sequence point mutation or sequence point deletion. The SPD_1741 gene comprises itself and homologous genes or alleles. The invention also disclosesa preparation method of the streptococcus pneumonia deficient in the LytR function, a use of the LytR protein as an antibiotic target of streptococcus pneumonia or other bacteria and a use of the streptococcus pneumoniae deficient in the LytR function as a vaccine for preventing, reducing or treating streptococcus pneumonia infection. Through use of the entire deletion, partial deletion, sequencepoint mutation or sequence point deletion of the SPD_1741 gene and its homologous genes or alleles, the expression of the SPD_01741 gene and its encoded product is disrupted or inhibited so that theLytR function defects caused by SPD_01741 gene function production or activity loss is disrupted or destroyed.

Description

technical field [0001] The present invention relates to the field of medical biotechnology, in particular to a Streptococcus pneumoniae with LytR functional deficiency, a vaccine and its preparation method and application, and also relates to an antibiotic target acting on Streptococcus pneumoniae and Streptococcus pneumoniae with LytR functional deficiency as a preventive Vaccines for pneumococcal infection. Background technique [0002] Streptococcus pneumoniae (S.pn) usually resides on the mucosal surface of the human nasopharynx. It is an opportunistic pathogen that can cause pneumonia, sepsis, meningitis or otitis media. The total number of deaths due to Streptococcus pneumoniae infection is as high as 3 to 5 million people in the world every year, especially children and the elderly. Among them, the annual incidence of children under 5 years old is as high as 1 million, and the mortality rate is as high as 11%. [0003] In the treatment of Streptococcus pneumoniae inf...

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Problems solved by technology

[0006] The present invention intends to provide a Streptococcus pneumoniae with LytR functional deficiency and its preparation method, and an antibiotic target for Streptococcus pneumoniae, so as to solve the problems of drug resistance and expensive vaccines that are difficult to popularize in the treatment of Streptococcus pneumoniae infection

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