A kind of method for quantitative determination of oil in culture medium
A quantitative measurement and quality technology, applied in the field of fermentation, can solve the problem of no quantitative detection method reported in the literature.
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Embodiment 1
[0062] The relaxation time analysis of embodiment 1, several components
[0063] In this example, relaxation time analysis of several components was performed.
[0064] The components in the culture medium are complex. First, the main components in the culture medium, water, oil (soybean oil), soybean powder, and germ powder, are tested separately to explore their characteristics and provide a basis for their effective analysis in the culture medium. The process is as follows: Measure water, oil, soybean powder, and germ powder of different qualities into test tubes, but the height in the test tube is not higher than 2cm, and then use soybean oil to find the center frequency and parameter settings. The quality of each component see figure 1 legend for .
[0065] Test results such as figure 1 . The relaxation time of water is about 2500ms, the relaxation time of oil is about 100ms, and the relaxation time of soybean powder and germ powder is about 0.1-1ms, 2-15ms, 20-400ms,...
Embodiment 2
[0066] Embodiment 2, Determination of the amount of characterized oil in the detection of low-field nuclear magnetic resonance technology
[0067] In the present embodiment, carry out the determination of the amount of characteristic oil in the detection of low-field nuclear magnetic resonance technology, the process is as follows:
[0068] Select pure water, pure oil and culture medium as the substance to determine the response value and inversion peak area, which is the amount of oil; take 0.5ml, 1.0ml, 1.5ml, 2.0ml, 2.5ml pure water to the test tube respectively, and take 0.6g, 0.9g, 1.4g, 1.9g, 2.6g soybean oil to the test tube, get 2ml oil content of 0%, 1%, 2%, 5%, 8%, 10% of the culture medium (not centrifuged ) to the detection test tube, and then carry out low-field nuclear magnetic resonance detection; water volume and response value and corresponding oil peak area do correlation fitting, the result is as follows figure 2 A. The correlation between the peak area an...
Embodiment 3
[0070] Embodiment 3, component is analyzed for the influence of determination
[0071] In the whole process from the preparation of the culture medium to the detection of low-field NMR, many operations or components may cause errors. In order to determine the influence of each substance on the oil peak area, the present inventors conducted in-depth analysis and research on each substance. Statistical analysis was performed on the research data, and some measurement results are shown in Table 3.
[0072] Table 3. Oil peak area affected by each component
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[0074]
[0075] *Using each group as a variable, set up six gradients, and do 3 parallels for each gradient. Low-field NMR detection was performed on the culture medium with each component as a variable.
[0076] The inventors found that with the increase of the content of the medium components, under the influence of each component, the oil peak area did not show regular changes. By calculating the coeffici...
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