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Forest leech antithrombotic peptide sylvestin and its gene and application

An anti-thrombotic peptide and anti-thrombotic technology, applied in the field of biomedicine, can solve problems such as high bleeding incidence, and achieve the effects of anti-thrombotic inhibition of acute cerebral ischemia, significant inhibition of acute cerebral ischemia, and convenient artificial synthesis.

Active Publication Date: 2021-06-11
云南中科龙津生物科技有限公司
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  • Abstract
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Problems solved by technology

The target of t-PA is plasminogen, which leads to a higher incidence of bleeding

Method used

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  • Forest leech antithrombotic peptide sylvestin and its gene and application
  • Forest leech antithrombotic peptide sylvestin and its gene and application
  • Forest leech antithrombotic peptide sylvestin and its gene and application

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Embodiment Construction

[0025] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0026] Clone of forest leech antithrombotic peptide gene:

[0027] 1. Forest leech total RNA extraction:

[0028] (1) Quickly take out many leeches from the liquid nitrogen, cut off the front end of the leeches (including the leech mouth and pharynx) with scissors, with a total weight of about 100 mg, put them into a mortar, add liquid nitrogen and grind them thoroughly. After being ground into a powder, 1 ml of Trizol extraction buffer (Invitrogen) was added and co-grinded with liquid nitrogen. After the Trizol is dissolved, suck out all the liquid in the mortar and put it into a 1.5ml centrifuge tube, and let it stand at room temperature for 5 minutes.

[0029] (2) Add 200 μL of chloroform, shake vigorously for 15 seconds, and place at room temperature for 5 minutes; centrifuge at 12,000 g for 10 minutes at 4°C; ...

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Abstract

The invention relates to a forest leech (Haemadipsa sylvestris) antithrombotic peptide Sylvestin and its gene and application, belonging to the technical field of biomedicine. The forest leech antithrombotic peptide Sylvestin is a single-chain polypeptide encoded by the forest leech analgesic peptide gene, with a molecular weight of 4790.5 Daltons and an isoelectric point of 6.28. The leech antithrombotic peptide Sylvestin consists of the amino acid sequence shown in SEQ ID NO:1. The gene encoding the leech antithrombotic peptide Sylvestin consists of the nucleotide sequence shown in SEQ ID NO:2. The leech antithrombotic peptide Sylvestin of the present invention can inhibit FXIIa and kallikrein, and has extremely significant antithrombotic and acute cerebral hemorrhage effects; the antithrombotic peptide has simple structure, convenient artificial synthesis, strong antithrombotic activity, and can be used as a preparation for FXIIa , kallikrein inhibitors and anti-thrombotic and anti-acute cerebral hemorrhage drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an antithrombotic peptide Sylvestin from Haemadipsasylvestris and its gene and application. Background technique [0002] Cerebrovascular disease is one of the main causes of death among middle-aged and elderly people in my country, and it is also one of the priorities of global health strategy research. Among cerebrovascular diseases, the incidence of acute ischemic diseases ranks first, and has the characteristics of high disability rate, high mortality rate and high recurrence rate. There are more than 1.5 million stroke deaths per year worldwide, which is increasing year by year with the increase of the elderly population, which seriously threatens human life, health and quality of life. Therefore, the study of drugs for the treatment of acute cerebral ischemia has a huge social demand and important social significance. [0003] The main cause of acute cereb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/815C12N15/15A61K38/58A61P7/02A61P9/10
CPCC12N9/6445C12N9/6451C12Y304/21038C07K14/815A61K38/00A61K38/58A61P7/02A61P9/10
Inventor 赖仞刘伟慧龙承波吕秋敏
Owner 云南中科龙津生物科技有限公司
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