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Immunoassay method as well as system and kit for identifying immunoassay

A technology of immunoassay and chemiluminescence immunity, applied in the field of light-induced chemiluminescence, which can solve the problems of limited detection range and unsustainable increase of signal

Active Publication Date: 2018-06-12
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] On the other hand, because of the high dose-hook effect, when the sample concentration increases to a certain value, the signal cannot continue to increase, which limits the detection range

Method used

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  • Immunoassay method as well as system and kit for identifying immunoassay
  • Immunoassay method as well as system and kit for identifying immunoassay
  • Immunoassay method as well as system and kit for identifying immunoassay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] Example 1 Detection of human chorionic gonadotropin and β subunit (HCG+β) in human serum samples to verify the effectiveness of the method of the present invention

[0144] Human chorionic gonadotropin and β subunit (HCG+β) detection kit (photo-induced chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect human chorionic gonadotropin and β in serum samples Subunit (HCG+β) content. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0145] Get 18 routine HCG+β concentration patient serum samples by Roche detection (exceed detection limit sample and carry out dilution detection), carry out conventional method and the method detection of the present invention respectively,

[0146] Routine detection method: the sample to be tested, calibrator, reagent 1 (luminescent antibody, tha...

Embodiment 2

[0154] Embodiment 2: detection of ferritin (Ferr) in the sample verifies the validity of the method of the present invention

[0155] The ferritin (Ferr) detection kit (chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the content of ferritin (purchased from Fitzgerald, Catalog No: 30-AF10) in the sample. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0156] Calibrator 1-Calibrator 6: Samples with known concentrations in conventional kits, the concentration is much lower than that of HOOK samples, and a standard curve is used to calculate the concentration of the analyte.

[0157] Other components to be used: LiCA universal solution (streptavidin-labeled photosensitive particles), which is an auxiliary reagent for the production of light-induced chemiluminescence analysis sys...

Embodiment 3

[0167] Example 3: C-peptide (CP) in the detection sample verifies the effectiveness of the method of the present invention

[0168] The C-peptide (CP) detection kit (chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the content of C-peptide (purchased from Fitzgerald, Catalog No: 30-AC96) in the sample. The kit includes calibrator 1-calibrator 6, reagent 1 (luminescent antibody, that is, antibody-coated luminescent particles), and reagent 2 (biotin-labeled antibody, that is, biotin-labeled antibody).

[0169] Calibrator 1-Calibrator 6: Samples with known concentrations in conventional kits, the concentration is much lower than that of HOOK samples, and a standard curve is used to calculate the concentration of the analyte.

[0170] The high-concentration C-peptide antigen is serially diluted, and the concentration values ​​of samples containing different concentrations of C-peptide are determined by conventional detection method...

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Abstract

The invention provides an immunoassay method which is characterized by comprising the following steps: (1) performing chemiluminiscence immunoreactions on a sample to be tested with antigen (or an antibody) to be tested, executing and recording a first reading and a second reading of chemiluminiscence, and recording a difference increase amplitude between the first reading and the second reading as A; (2) making a standard curve according to the increase amplitude A of two readings of a series of known standard substances of the target antibody (or the antibody) to be tested, wherein the concentrations of the standard substances are lower than that for an HOOK effect; (3) if the increase amplitude A of the two readings of the sample to be tested with the target antibody (or the antibody) to be tested is greater than the maximum value of the standard curve, testing the sample for another time after the sample is diluted. The invention further relates to a system and a kit for identifying immunoassay. The invention further relates to another immunoassay method, another system and another kit for identifying immunoassay.

Description

technical field [0001] The present invention relates to the technical field of light-activated chemiluminescence, in particular to an immunoassay method, a system for identifying an immunoassay, and a kit; another immunoassay method, another system for identifying an immunoassay system and another kit. Background technique [0002] Immunological detection is based on the principle of antigen-antibody specific reaction. Because it can use isotopes, enzymes, chemiluminescent substances, etc. to display or amplify the signal of the measured substance, it is often used to detect trace organisms such as proteins and hormones. active substance. [0003] Chemiluminescent immunoassay is a non-radioactive immunoassay technology that has developed rapidly in recent years. Its principle is to use chemiluminescent substances to amplify the signal, and use its luminous intensity to directly measure the immune binding process. This method has become an immunological method. One of the i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N21/76
CPCG01N21/76G01N33/5761G01N33/5764G01N33/5767G01N33/58G01N33/6854G01N33/6893G01N33/76
Inventor 杨阳赵卫国张向辉
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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