A kind of Sequoia substratum and culture method thereof
A technology of culture medium and cultivation method, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of low survival rate, achieve high survival rate and improve the effect of survival rate
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[0071] A kind of cultivation method of North American Sequoia, such as figure 1 shown, including the following steps:
[0072] S1, get the material, select the Sequoia twigs that were born in the current year as raw materials, and inoculate them into MS culture medium after disinfection and cleaning, and grow aseptic seedlings from the axillary buds after 20-22 days;
[0073] S2, proliferating culture, inoculating the sterile vaccine into the proliferating medium for proliferating culture. After 23-27 days of culture, a sterile vaccine is formed;
[0074] S3, strong seedlings, put the sterile seedlings formed after multiplication into the strong seedling medium for strong seedling culture, and form tissue culture seedlings after 2-3 weeks of cultivation, the strong seedling medium adopts 1L mother liquor and 20mL0.01 concentration The NAA composition;
[0075] S4, rooting culture, the tissue culture seedling is inoculated on the rooting medium and is cultivated to form the ...
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